| Analysis of survival rates and cellular fatty acid profiles of Listeria monocytogenes treated with supercritical carbon dioxide under the influence of cosolvents. | |
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MedLine Citation:
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PMID: 18565606 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present study, we identified several process variables that significantly affect the efficiency of supercritical carbon dioxide inactivation of the food-borne pathogen Listeria monocytogenes. Treatment with SC-CO(2) completely disabled the colony-forming activity of the cells (8-log reduction) within specific treatment time (10-50 min), pressure (80-150 bar), and temperature ranges (35-45 degrees C). Microorganism inactivation rates increased proportionally with pressure and temperature, but the inactivation rate decreased significantly when cells were suspended in phosphate-buffered saline rather than in physiological saline. Additionally, when the microbial cell suspension was 80-100% (w/w) of water, the SC-CO(2)-mediated reduction in CFU ml(-1) was 4-8 log higher at the same treatment conditions than in typical cell suspensions (a water content of 800-4000% [w/w]) or dry preparations that had only 2-10% (w/w) of water. The addition of a fatty acid, oleic acid, decreased the effectiveness of the microbial inactivation by SC-CO(2), but the addition of a surfactant, sucrose monolaurate, increased the effectiveness. Therefore, cosolvents for SC-CO(2), including water, a fatty acid, and a surfactant in this study, were found to greatly influence on the inactivation effectiveness. The extraction of cellular substances, such as nucleic acid- and protein-like materials and fatty acids, was monitored by spectrophotometry and GC/MS and increased with SC-CO(2) treatment time. Additionally, using scanning and transmission electron microscopies, we investigated morphological changes in the SC-CO(2)-treated cells. The effects of the variables we have described herein represent a significant contribution to our current knowledge of this method of inactivating food-borne pathogens. |
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Authors:
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Soo Rin Kim; Hee Jung Park; Do Seong Yim; Hee Tack Kim; In-Geol Choi; Kyoung Heon Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-16 |
Journal Detail:
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Title: Journal of microbiological methods Volume: 75 ISSN: 0167-7012 ISO Abbreviation: J. Microbiol. Methods Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-12 Completed Date: 2008-10-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8306883 Medline TA: J Microbiol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 47-54 Citation Subset: IM |
Affiliation:
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School of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbon Dioxide
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pharmacology* Fatty Acids / metabolism* Food Microbiology* Food Preservation / methods* Listeria monocytogenes / cytology, drug effects*, metabolism Microbial Viability / drug effects* Pressure Solvents / pharmacology* Temperature |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Solvents; 124-38-9/Carbon Dioxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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