Document Detail


Analysis of sphingolipids in potatoes (Solanum tuberosum L.) and sweet potatoes (Ipomoea batatas (L.) Lam.) by reversed phase high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS).
MedLine Citation:
PMID:  17103377     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ceramides and glucocerebrosides of potatoes (Solanum tuberosum L.) and sweet potatoes (Ipomoea batatas (L.) Lam.) were analyzed using RP-HPLC-ESI-MS/MS. Ceramides and glucocerebrosides containing the three different long-chain bases 4,8-sphingadienine (d18:2(delta4,delta8)), 4-hydroxy-8-sphingenine (t18:1(delta8)), and 8-sphingenine (d18:1(delta8)) acylated to saturated and unsaturated hydroxy- and nonhydroxy fatty acids with 16-26 carbon atoms were detected. For ceramides and glucocerebrosides 4,8-sphingadienine (d18:2(delta4,delta8)) was found as the major long-chain base, with lesser amounts of 4-hydroxy-8-sphingenine (t18:1(delta8)) and 8-sphingenine (d18:1(delta8)). 2-(Alpha-)hydroxypalmitic acid (C16:0h) was the major fatty acid, which was found to be acylated to the long-chain bases. For quantification of these compounds, an RP-HPLC-ESI-MS/MS method with an "echo-peak"-technique simulating internal standard injection was developed. The analyzed samples of potatoes and sweet potatoes showed amounts of approximately 0.1-8 microg/kg single ceramides and amounts up to 500 microg/kg glucocerebrosides, with C16:0h-glucosyl-4,8-sphingadienine as the major component.
Authors:
Nana Bartke; Anne Fischbeck; Hans-Ulrich Humpf
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular nutrition & food research     Volume:  50     ISSN:  1613-4125     ISO Abbreviation:  Mol Nutr Food Res     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-12-25     Completed Date:  2007-03-12     Revised Date:  2007-03-22    
Medline Journal Info:
Nlm Unique ID:  101231818     Medline TA:  Mol Nutr Food Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1201-11     Citation Subset:  IM    
Affiliation:
Institut für Lebensmittelchemie, Westfälische Wilhelms-Universität Münster, Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Ceramides / analysis
Chromatography, High Pressure Liquid*
Glucosylceramides / analysis
Ipomoea batatas / chemistry*
Plant Tubers / chemistry*
Solanum tuberosum / chemistry*
Spectrometry, Mass, Electrospray Ionization*
Sphingolipids / analysis*
Chemical
Reg. No./Substance:
0/Ceramides; 0/Glucosylceramides; 0/Sphingolipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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