| Analysis of several iridoid and indole precursors of terpenoid indole alkaloids with a single HPLC run. | |
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MedLine Citation:
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PMID: 17252445 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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An isocratic HPLC system is described which allows the separation of the iridoid and indole precursors of terpenoid indole alkaloids, which are present in a single crude extract. The system consists of a column of LiChrospher 60 RP select B 5 microm, 250 x 4 mm (Merck) with an eluent of 1% formic acid-acetonitrile-trichloroacetic acid (100:10:0.25, v:v:w) at a flow of 1.2 ml/min. In the suspension cultures of Catharanthus roseus secologanin and tryptophan were detected. in the cultures of Tabernaemontana divaricata loganin, tryptophan, and tryptamine accumulated. |
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Authors:
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D Dagnino; J Schripsema; R Verpoorte |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Planta medica Volume: 62 ISSN: 0032-0943 ISO Abbreviation: Planta Med. Publication Date: 1996 Jun |
Date Detail:
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Created Date: 2007-01-25 Completed Date: 2007-08-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0066751 Medline TA: Planta Med Country: Germany |
Other Details:
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Languages: eng Pagination: 278-80 Citation Subset: - |
Affiliation:
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Division of Pharmacognosy, Center for Biopharmaceutical Sciences, Leiden University, The Netherlands. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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