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Analysis of several iridoid and indole precursors of terpenoid indole alkaloids with a single HPLC run.
MedLine Citation:
PMID:  17252445     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
An isocratic HPLC system is described which allows the separation of the iridoid and indole precursors of terpenoid indole alkaloids, which are present in a single crude extract. The system consists of a column of LiChrospher 60 RP select B 5 microm, 250 x 4 mm (Merck) with an eluent of 1% formic acid-acetonitrile-trichloroacetic acid (100:10:0.25, v:v:w) at a flow of 1.2 ml/min. In the suspension cultures of Catharanthus roseus secologanin and tryptophan were detected. in the cultures of Tabernaemontana divaricata loganin, tryptophan, and tryptamine accumulated.
Authors:
D Dagnino; J Schripsema; R Verpoorte
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Planta medica     Volume:  62     ISSN:  0032-0943     ISO Abbreviation:  Planta Med.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  2007-01-25     Completed Date:  2007-08-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0066751     Medline TA:  Planta Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  278-80     Citation Subset:  -    
Affiliation:
Division of Pharmacognosy, Center for Biopharmaceutical Sciences, Leiden University, The Netherlands.
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