Document Detail

Analysis of sequential aliquots of hypertonic saline solution-induced sputum from clinically stable patients with cystic fibrosis.
MedLine Citation:
PMID:  12628880     Owner:  NLM     Status:  MEDLINE    
STUDY OBJECTIVES: Sputum induction (SI) is a noninvasive tool for sampling inflamed airways. The purpose of this study was to determine the optimal duration of collection in patients with cystic fibrosis (CF). The hypothesis was that the duration of SI collection would quantitatively and qualitatively alter the content of the induced sputum. METHODS: In 10 clinically stable patients with CF (mean +/- SD age, 28 +/- 7 years; mean FEV(1), 2.6 +/- 0.7 L), SI was performed with 3% hypertonic saline solution at five time points over 20 min. RESULTS: SI was well tolerated, with an average maximum fall in FEV(1) of 7 +/- 7%. The sample volumes, urea concentrations, interleukin-8 concentrations, total cell counts, and nonsquamous cell counts remained constant (p > 0.05). The percentage of neutrophils decreased from 89 +/- 5% to 86 +/- 4% (p = 0.03), and the percentage of alveolar macrophages increased 5 +/- 2% to 8 +/- 4% (p < 0.01). The mean quantitative microbiological counts of nonmucoid Pseudomonas aeruginosa and Staphylococcus aureus decreased over the 20-min time period each by half a log (p = 0.05 and p < 0.01, respectively). Surfactant protein-A concentration increased from 1.6 +/- 0.3 to 2.4 +/- 0.4 ng/mL (log(10); p < 0.001). CONCLUSIONS: We conclude that aliquots of induced sputum are similar in clinically stable patients with CF during 4-min intervals, although there is more alveolar sampling after 20 min. When induced-sputum samples are fractionated for research monitoring of inflammatory or microbiologic indexes, power calculations accounting for these variations over time are required.
Moira L Aitken; Kelly E Greene; Mark R Tonelli; Jane L Burns; Julia C Emerson; Christopher H Goss; Ronald L Gibson
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chest     Volume:  123     ISSN:  0012-3692     ISO Abbreviation:  Chest     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-11     Completed Date:  2003-04-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0231335     Medline TA:  Chest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  792-9     Citation Subset:  AIM; IM    
Department of Medicine, Division of Pulmonary and Critical Care, University of Washington, Seattle 98195, USA.
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MeSH Terms
Biological Markers
Cell Count
Colony Count, Microbial
Cystic Fibrosis / diagnosis*
Interleukin-8 / metabolism
Pilot Projects
Pulmonary Surfactant-Associated Proteins / metabolism
Regression Analysis
Saline Solution, Hypertonic
Specimen Handling / methods*
Sputum / chemistry*,  cytology
Time Factors
Urea / metabolism
Reg. No./Substance:
0/Biological Markers; 0/Interleukin-8; 0/Pulmonary Surfactant-Associated Proteins; 0/Saline Solution, Hypertonic; 57-13-6/Urea

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