Document Detail


Analysis of residual trifluoroacetic acid in a phosphate-buffered saline matrix by ion chromatography with suppressed conductivity detection.
MedLine Citation:
PMID:  11452994     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
As part of the formulation of a cell-based pharmaceutical product, cells were harvested from mice and incubated in a cocktail containing cell culture media and high levels of trifluoroacetic acid (TFA). The cells were washed with a phosphate-buffered saline solution to remove residual cell culture media and other reagents before the cells were infused back into the mice from which they originated. Because of the potentially toxic nature of the TFA, the cells were washed multiple times and the final wash was monitored for residual TFA in order to demonstrate the efficient removal of the reagent before the cell product could be reintroduced into the test animal. This report describes the method that was developed incorporating anion-exchange chromatography with suppressed conductivity detection for the analysis of residual TFA (down to 50 ng/ml) in the presence of high concentrations of phosphate and chloride interferences. The ultimate sensitivity of the method was improved by selectively removing halide anions using a silver cartridge before sample analysis. The method proved to be rugged and reproducible enough to be validated and used to monitor residual TFA levels in cell washes in support of an acute toxicological study. Results demonstrating the method's sensitivity, selectivity, precision and linearity were reported.
Authors:
P N Fernando; M A McLean; I N Egwu; E deGuzman; C Weyker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  920     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-07-16     Completed Date:  2001-12-04     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  155-62     Citation Subset:  IM    
Affiliation:
Pharmacia Corporation, Skokie, IL 60077, USA.
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MeSH Terms
Descriptor/Qualifier:
Buffers
Chromatography, Ion Exchange / methods*
Phosphates / chemistry*
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Sodium Chloride / chemistry*
Trifluoroacetic Acid / analysis*
Chemical
Reg. No./Substance:
0/Buffers; 0/Phosphates; 76-05-1/Trifluoroacetic Acid; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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