Document Detail

Analysis of the replication mode of double minutes using the PCC technique combined with BrdUrd labeling.
MedLine Citation:
PMID:  3219916     Owner:  NLM     Status:  MEDLINE    
A cultured line of neuroblastoma cells (NB) was found to contain double minute chromosomes (DMs) DMs have been reported to be acentric and, therefore, to be segregated randomly into daughter cells without separating their sister elements. When NB cells were fused with Chinese hamster metaphase cells, prematurely condensed chromosomes (PCCs) were induced. DMs seen together with G2 PCCs appeared to be closely paired, dot-like structures resembling DMs observable in metaphase cells. In contrast, DMs in G1 cells showed a tendency to become single as the stage progressed so that the majority of DMs in late G1 cells were actually no longer double. DMs in S-phase cells, however, again appeared double. These results clearly indicate why DMs are invariably double and never assume a quadruple configuration in metaphase cells in spite of their non-disjunctional segregation at anaphase. Such a characteristic mode of DM replication was further confirmed by a 5-bromo-2'-deoxyuridine (BrdUrd) labeling experiment: when NB cells were exposed to BrdUrd for two successive rounds of DNA replication prior to PCC induction, half of the resulting single G1 minutes as well as G1 PCCs stained dark and the other half stained light after staining for sister chromatid differentiation.
S Takayama; Y Uwaike
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chromosoma     Volume:  97     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1989-03-22     Completed Date:  1989-03-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  198-203     Citation Subset:  IM    
Biological Laboratory, School of Science, Kwansei Gakuin University, Nishinomiya, Japan.
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MeSH Terms
Bromodeoxyuridine / diagnostic use*
Chromosome Aberrations*
DNA Replication*
Neuroblastoma / genetics
Tumor Cells, Cultured
Reg. No./Substance:

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