Document Detail

Analysis of the relationship between age of larvae at mutagen treatment and frequency and size of spots in the wing somatic mutation and recombination test in Drosophila melanogaster.
MedLine Citation:
PMID:  7875256     Owner:  NLM     Status:  MEDLINE    
The relationship between the induction of mutant clones and the time of mutagen treatment was studied in the somatic mutation and recombination test (SMART) in wing cells of Drosophila melanogaster. Larvae trans-heterozygous for the recessive marker mutations multiple wing hairs (mwh) and flare (flr) were produced. Batches of these larvae were then treated with mutagen at different ages spanning all three larval instars. Methyl methanesulfonate was fed acutely for 2 h by immersing the larvae in a solution of the mutagen mixed with powdered cellulose. Wings of the surviving adult flies were mounted and scored for the presence of spots. The frequency and size of single and twin spots were recorded separately. Twin spots are produced exclusively by mitotic recombination, whereas single spots can result from various types of mutational and exchange events. There exists a clear correlation between time of induction and frequency as well as size of the single spots. In young larvae only few but very large spots are induced, whereas in older larvae the frequencies are considerably increased but the sizes are smaller. The twin spots show a different relationship. Practically no twin spots are found in very young and in very old larvae. The results demonstrate that in the wing spot test the optimal age of the larvae for mutagen treatment is 72 h.
U Graf
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experientia     Volume:  51     ISSN:  0014-4754     ISO Abbreviation:  Experientia     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-03-31     Completed Date:  1995-03-31     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376547     Medline TA:  Experientia     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  168-73     Citation Subset:  IM    
Institute of Toxicology, Swiss Federal Institute of Technology (ETH), Schwerzenbach.
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MeSH Terms
Cell Division / drug effects
Drosophila melanogaster / genetics*,  growth & development
Larva / genetics
Methyl Methanesulfonate / pharmacology
Time Factors
Wing / cytology,  growth & development*
Reg. No./Substance:
66-27-3/Methyl Methanesulfonate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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