| Analysis of regulatory factors for urea synthesis by isolated perfused rat liver. II. Comparison of urea synthesis in livers of rats subjected to different dietary conditions. | |
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MedLine Citation:
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PMID: 1150635 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Capacities for urea synthesis and amino acid patterns in the perfused livers isolated from rats fed low and high-protein diets were compared. Urea formation with amjonium chlorode as the nitrogen source in perfused livers isolated from rats fed on a 70% casein diet was rapid and the efficiency of conversion of ammonia to urea was 97.9%. However, that in livers isolated from rats fed on a 5% casein diet was much slower and the efficiency of conversion of ammonia to urea was only 36.1%. The ratios of the rate of urea formation from ammonium chloride to activity of ornithine transcarbamylase [EC 2.1.3.3.] in the perfused livers of rats fed on 5 and 70% casein diets were calculated. The ratio of the former condition was much lower than that of the latter. The ratios reached nearly the same level by the addition of ornithine and N-acetylglutamate, the addition of which to the perfusate caused marked elevation of the ratios in both cases. In the perfused livers from rats fed on a 5% casein diet a considerable portion of the ammonia added to the perfusate was fixed into an amino ro an amide group of amino acids such as alamin, aspartate, and glutamine. On the other hand, in the perfused livers from rats fed on a 70% casein diet most of the ammonia added was converted to urea. The regulation of urea synthesis and the relation between anabolism and catabolism of amino acids in rat livers subjected to different dietary conditions were compared. |
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Authors:
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T Saheki; M Tsuda; T Tanaka; N katunuma |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biochemistry Volume: 77 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1975 Mar |
Date Detail:
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Created Date: 1975-11-07 Completed Date: 1975-11-07 Revised Date: 2007-12-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 671-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Ammonium Chloride / metabolism Animals Diet* Dietary Proteins / metabolism Glutamates / pharmacology Liver / analysis, enzymology, metabolism* Male Ornithine / pharmacology Ornithine Carbamoyltransferase / metabolism Rats Urea / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Dietary Proteins; 0/Glutamates; 12125-02-9/Ammonium Chloride; 57-13-6/Urea; 7006-33-9/Ornithine; EC 2.1.3.3/Ornithine Carbamoyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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