| Analysis of regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in a somatic cell mutant auxotrophic for mevalonate. | |
| | |
MedLine Citation:
|
PMID: 7085625 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Regulation of 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase activity and synthesis by 25-hydroxycholesterol is aberrant in a somatic cell mutant of the Chinese hamster ovary K1 cell auxotrophic for mevalonate by virtue of a defect in HMG-CoA synthase activity. Normal regulation of HMG-CoA reductase activity and synthesis in this mutant by 25-hydroxycholesterol can be restored by simultaneous incubation with a small (0.4 mM) mevalonate supplement. Normal regulation of HMG-CoA reductase is also observed in a revertant of the mutant cell with normal HMG-CoA synthase activity. |
| | |
Authors:
|
M Sinensky; R Torget; R Schnitzer-Polokoff; P A Edwards |
Related Documents
:
|
6835225 - Sister-chromatid exchange formation at supra-optimal growth temperatures. 178625 - The induction of sv40-transformed chinese hamster and mouse kidney cells by mitomycin c. 2916165 - Single-step selection of mammalian cell mutants deficient in ctp synthetase. 106395 - Identification of 7,12-dimethylbenz[a]anthracene metabolites that lead to mutagenesis i... 3280545 - A rapid fluorometric dna assay for the measurement of cell density and proliferation in... 1018045 - Circus movements and blebbing locomotion in dissociated embryonic cells of an amphibian... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 257 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1982 Jul |
Date Detail:
|
Created Date: 1982-08-26 Completed Date: 1982-08-26 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 7284-6 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line Cricetinae Cricetulus Female Hydroxycholesterols / pharmacology Hydroxymethylglutaryl CoA Reductases / biosynthesis*, isolation & purification Kinetics Mevalonic Acid / metabolism* Molecular Weight Ovary |
| Grant Support | |
ID/Acronym/Agency:
|
CA 15794/CA/NCI NIH HHS; GM24732/GM/NIGMS NIH HHS; HD 02080/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Hydroxycholesterols; 150-97-0/Mevalonic Acid; 2140-46-7/25-hydroxycholesterol; EC 1.1.1.-/Hydroxymethylglutaryl CoA Reductases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: DNA primase activity from human lymphocytes. Synthesis of oligoribonucleotides that prime DNA synthe...
Next Document: Dopamine-derived tetrahydroisoquinolines. Novel inhibitors of dihydropteridine reductase.