Document Detail


Analysis of recombinants in female mouse meiosis.
MedLine Citation:
PMID:  23138942     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During meiosis, homologous chromosomes (homologs) undergo recombinational interactions, resulting in the formation of crossovers (COs) or noncrossovers (NCOs). Both COs and NCOs are initiated by the same event: programmed double-strand DNA breaks (DSBs), which occur preferentially at hotspots throughout the genome. COs contribute to the genetic diversity of gametes and are needed to promote proper meiotic chromosome segregation. Accordingly, their formation is tightly controlled. In the mouse, the sites of preferred CO formation differ between male and female chromosomes, both on a regional level and on the level of individual hotspots. Sperm typing using (half-sided) allele-specific PCR has proven a powerful technique to characterize COs and all detectable NCOs at hotspots on male human and mouse chromosomes. In contrast, very little is known about the properties of hotspots in female meiosis. This chapter describes an adaptation of sperm typing to analyze recombinants in a hotspot, using DNA isolated from an ovary cell suspension enriched for oocytes.
Authors:
Esther de Boer; Maria Jasin; Scott Keeney
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  957     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-09     Completed Date:  2013-04-12     Revised Date:  2013-04-22    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  19-45     Citation Subset:  IM    
Affiliation:
Institut de Génétique et Microbiologie, Université Paris-Sud, Orsay, France.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Animals
Cytogenetic Analysis / methods*
DNA / genetics,  isolation & purification
DNA Primers / genetics
Female
Fertilization in Vitro
Male
Meiosis / genetics*
Mice
Ovary / cytology,  metabolism
Polymerase Chain Reaction
Recombination, Genetic*
Spermatozoa / cytology,  metabolism
Grant Support
ID/Acronym/Agency:
R01 HD053855/HD/NICHD NIH HHS; R01 HD53855/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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