Document Detail

Analysis of ras DNA sequences in rat renal cell carcinoma.
MedLine Citation:
PMID:  1910479     Owner:  NLM     Status:  MEDLINE    
The DNA sequences for Ha-, Ki-, and N-ras were determined in six cell lines derived from independent rat hereditary renal cell carcinomas (RCC). Genomic regions encompassing codons 12, 13, and 61 of Ha-ras, Ki-ras, and N-ras, and codon 117 of Ha-ras were PCR amplified and directly sequenced. The DNA sequences of Ha-ras and Ki-ras were normal in all lines tested, as were the codon 12 and 61 sequences of N-ras. However, DNA sequence variations that could code for amino acid substitutions were observed in codons 13, 14, and 18 of N-ras in all the lines. The codon 13 Gly----Val alteration observed was consistent with activating N-ras mutations previously reported. When normal kidney DNA from rats with the hereditary tumor syndrome was sequenced, the same N-ras sequence variations observed in the tumor lines were found. DNA from outbred Long-Evans and inbred Fischer rats also had the altered N-ras sequences. The variant N-ras sequence was not observed in PCR-amplified N-ras cDNA from the RCC lines. Thus, tumor-associated activation of ras oncogene appears to be an infrequent event in spontaneous rat RCC. In addition, these data indicate that rats contain an N-ras DNA polymorphism that appears to be a species-specific anomaly.
L Recio; S C Lane; J Ginsler; C Walker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular carcinogenesis     Volume:  4     ISSN:  0899-1987     ISO Abbreviation:  Mol. Carcinog.     Publication Date:  1991  
Date Detail:
Created Date:  1991-11-14     Completed Date:  1991-11-14     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8811105     Medline TA:  Mol Carcinog     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  350-3     Citation Subset:  IM    
Chemical Industry Institute of Toxicology, Research Triangle Park, North Carolina 27709.
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MeSH Terms
Base Sequence
Carcinoma, Renal Cell / genetics*
DNA Mutational Analysis
DNA, Neoplasm / genetics
Genes, ras*
Molecular Sequence Data
Oligonucleotides / chemistry
Polymerase Chain Reaction
Polymorphism, Genetic
Proto-Oncogene Proteins p21(ras) / genetics*
Tumor Cells, Cultured
Reg. No./Substance:
0/DNA, Neoplasm; 0/Oligonucleotides; EC Proteins p21(ras)

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