| Analysis of ras DNA sequences in rat renal cell carcinoma. | |
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MedLine Citation:
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PMID: 1910479 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The DNA sequences for Ha-, Ki-, and N-ras were determined in six cell lines derived from independent rat hereditary renal cell carcinomas (RCC). Genomic regions encompassing codons 12, 13, and 61 of Ha-ras, Ki-ras, and N-ras, and codon 117 of Ha-ras were PCR amplified and directly sequenced. The DNA sequences of Ha-ras and Ki-ras were normal in all lines tested, as were the codon 12 and 61 sequences of N-ras. However, DNA sequence variations that could code for amino acid substitutions were observed in codons 13, 14, and 18 of N-ras in all the lines. The codon 13 Gly----Val alteration observed was consistent with activating N-ras mutations previously reported. When normal kidney DNA from rats with the hereditary tumor syndrome was sequenced, the same N-ras sequence variations observed in the tumor lines were found. DNA from outbred Long-Evans and inbred Fischer rats also had the altered N-ras sequences. The variant N-ras sequence was not observed in PCR-amplified N-ras cDNA from the RCC lines. Thus, tumor-associated activation of ras oncogene appears to be an infrequent event in spontaneous rat RCC. In addition, these data indicate that rats contain an N-ras DNA polymorphism that appears to be a species-specific anomaly. |
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Authors:
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L Recio; S C Lane; J Ginsler; C Walker |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular carcinogenesis Volume: 4 ISSN: 0899-1987 ISO Abbreviation: Mol. Carcinog. Publication Date: 1991 |
Date Detail:
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Created Date: 1991-11-14 Completed Date: 1991-11-14 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8811105 Medline TA: Mol Carcinog Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 350-3 Citation Subset: IM |
Affiliation:
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Chemical Industry Institute of Toxicology, Research Triangle Park, North Carolina 27709. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Carcinoma, Renal Cell / genetics* DNA Mutational Analysis DNA, Neoplasm / genetics Genes, ras* Molecular Sequence Data Oligonucleotides / chemistry Polymerase Chain Reaction Polymorphism, Genetic Proto-Oncogene Proteins p21(ras) / genetics* Rats Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Oligonucleotides; EC 3.6.5.2/Proto-Oncogene Proteins p21(ras) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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