Document Detail

Analysis of radiation-induced micronuclei by FISH using a combination of painting and centromeric DNA probes.
MedLine Citation:
PMID:  9106249     Owner:  NLM     Status:  MEDLINE    
In situ hybridization with whole chromosome painting probes (chromosome 1, 7, 11, 14, 17 and 21) in combination with a human pancentromeric alpha-satellite probe was used to analyse the presence of specific chromosomal material in micronuclei (MN) induced in human lymphocytes by ionizing radiation. The purpose was to investigate the nature of radiation-induced cytogenetic damage, especially to study whether the fraction of paint-positive MN is proportional to the relative DNA content of the respective chromosomes which might indicate a random breakage of chromosomes. Flow-sorted MN and MN in binucleated cells were analysed with the six chromosome specific painting probes. It was found that the fraction of paint-positive MN increased linearly with the DNA content of the respective chromosomes. About 13% radiation-induced MN in human lymphocytes were found to contain centromeric signals independent of the presence of specific chromosome painting signals. The data obtained on flow-sorted MN and MN in binucleated cells agreed well, indicating that flow-sorted MN can be used for studying their chromosomal content with the FISH technique. If it is assumed that the chromosomal content of MN reflects radiation-induced damage, then these results support a random model of radiation-induced cytogenetic damage in human lymphocytes for the six chromosomes studied.
C Fimognari; S Sauer-Nehls; H Braselmann; M Nüsse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutagenesis     Volume:  12     ISSN:  0267-8357     ISO Abbreviation:  Mutagenesis     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-06-12     Completed Date:  1997-06-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8707812     Medline TA:  Mutagenesis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  91-5     Citation Subset:  IM    
Università degli Studi di Bologna, Department of Pharmacology, Italy.
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MeSH Terms
Cell Nucleus / radiation effects*
Centromere / genetics*
Chromosome Banding / methods
Chromosomes, Human, Pair 1
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 21
Chromosomes, Human, Pair 7
DNA Probes*
In Situ Hybridization, Fluorescence / methods*
Micronucleus Tests / methods*
Reg. No./Substance:
0/DNA Probes

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