Document Detail

Analysis of purines in urinary calculi by high-performance liquid chromatography.
MedLine Citation:
PMID:  11067744     Owner:  NLM     Status:  MEDLINE    
A high-pressure liquid chromatography method has been developed for the analysis in urinary calculi of six purines: uric acid, 2, 8-dihydroxyadenine, xanthine, hypoxanthine, allopurinol, and oxypurinol. Separation was conducted isocratically on a reversed-phase column, using 50 mM phosphate buffer (pH 5.5) / methanol (97/3, v/v) as mobile phase. Limits of detection, depending on compound, ranged from 7 to 28 microg/g stone weight. Hitherto, no reports have appeared on other purines present with uric acid in stones, due to lack of a sensitive and specific analytical method. We have now found that all calculi with more than 4% uric acid also contained 1-methyluric and 7-methyluric acids and trace amounts of hypoxanthine, xanthine, and 2,8-dihydroxyadenine. Accurate identification and quantitation of purines in urinary calculi are important for the diagnosis of rare metabolic diseases leading to urolithiasis (xanthinuria, dihydroxyadeninuria), as well as for prevention of iatrogenic complications during treatment with allopurinol of uric acid urolithiasis. The method may be used for reference purposes in clinical laboratories and for research on the pathogenesis of urolithiasis in disorders of purine metabolism.
K Safranow; Z Machoy; K Ciechanowski
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical biochemistry     Volume:  286     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2001-03-08     Completed Date:  2001-05-31     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  224-30     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Department of Biochemistry and Chemistry, Pomeranian Academy of Medicine, Powstańców Wielkopolskich 72, Szczecin, PL-70-111, Poland.
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MeSH Terms
Chemistry Techniques, Analytical / methods
Chromatography, High Pressure Liquid / methods*
Purine-Pyrimidine Metabolism, Inborn Errors / complications,  diagnosis,  urine
Purines / urine*
Reproducibility of Results
Urinary Calculi / etiology,  urine*
Reg. No./Substance:

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