Document Detail


Analysis of the ptsH-ptsI-crr region in Escherichia coli K-12: nucleotide sequence of the ptsH gene.
MedLine Citation:
PMID:  2411636     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nucleotide sequence of an Escherichia coli DNA segment containing the ptsH gene and the first 162 nucleotides of the ptsI gene encoding, respectively, Hpr and enzyme I of the phosphoenolpyruvate-dependent glycose phosphotransferase system (PTS), was determined. The ptsH promoter was localized using the S1 mapping technique. A nucleotide sequence very similar to the consensus binding site for cAMP receptor protein was found in the -35 region of the ptsH promoter. The ptsH gene is transcribed in the same direction as the ptsI gene and the crr gene (encoding enzyme IIIGlc of the PTS). Analysis of the nucleotide sequence substantiates the notion that the ptsH-ptsI-crr genes constitute a polycistronic operon.
Authors:
H De Reuse; A Roy; A Danchin
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gene     Volume:  35     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1985  
Date Detail:
Created Date:  1985-10-03     Completed Date:  1985-10-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  199-207     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M10425
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosome Mapping
DNA, Bacterial / genetics*
Escherichia coli / enzymology,  genetics*
Genes
Genes, Bacterial*
Operon
Phosphoenolpyruvate Sugar Phosphotransferase System / genetics*
Plasmids
RNA, Bacterial / genetics
RNA, Messenger / genetics
Receptors, Cyclic AMP / genetics
Salmonella typhimurium / genetics
Transcription, Genetic
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/RNA, Bacterial; 0/RNA, Messenger; 0/Receptors, Cyclic AMP; EC 2.7.1.-/Phosphoenolpyruvate Sugar Phosphotransferase System

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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