Document Detail


Analysis of proteomic changes induced upon cellular differentiation of the human intestinal cell line Caco-2.
MedLine Citation:
PMID:  21492153     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The human intestinal cell line Caco-2 is a well-established model system to study cellular differentiation of human enterocytes of intestinal origin, because these cells have the capability to differentiate spontaneously into polarized cells with morphological and biochemical features of small intestinal enterocytes. Therefore, the cells are widely used as an in vitro model for the human intestinal barrier. In this study, a proteomic approach was used to identify the molecular marker of intestinal cellular differentiation. The proteome of proliferating Caco-2 cells was compared with that of fully differentiated cells. Two-dimensional gel analysis yielded 53 proteins that were differently regulated during the differentiation process. Pathway analysis conducted with those 34 proteins that were identified by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) analysis revealed subsets of proteins with common molecular and cellular function. It was shown that proteins involved in xenobiotic and drug metabolism as well as in lipid metabolism were upregulated upon cellular differentiation. In parallel, proteins associated with proliferation, cell growth and cancer were downregulated, reflecting the loss of the tumorigenic phenotype of the cells. Thus, the proteomic approach in combination with a literature-based pathway analysis yielded valuable information about the differentiation process of Caco-2 cells on the molecular level that contributes to the understanding of the development of colon cancer or inflammatory diseases such as ulcerative colitis - diseases associated with an imbalanced differentiation process of intestinal cells.
Authors:
Thorsten Buhrke; Imme Lengler; Alfonso Lampen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Development, growth & differentiation     Volume:  53     ISSN:  1440-169X     ISO Abbreviation:  Dev. Growth Differ.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0356504     Medline TA:  Dev Growth Differ     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  411-26     Citation Subset:  IM    
Copyright Information:
© 2011 Federal Institute for Risk Assessment, Germany. Journal compilation © 2011 Japanese Society of Developmental Biologists.
Affiliation:
Food Safety Department, Federal Institute for Risk Assessment, Thielallee 88-92, 14195 Berlin, Germany.
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