Document Detail

Analysis of the promoter element of the serum amyloid A gene and its interaction with constitutive and inducible nuclear factors from rabbit liver.
MedLine Citation:
PMID:  8268719     Owner:  NLM     Status:  MEDLINE    
In an effort to identify regulatory elements of the serum amyloid A (SAA) gene that play a major role in its expression under acute-phase conditions, we studied the expression of a set of chimeric SAA-chloramphenicol acetyltransferase (CAT) plasmids containing a progressively deleted upstream 5' sequence of the SAA gene. Two regulatory regions (-314 to -135 and -135 to -31) capable of driving cytokine-induced transcription have been identified. Gel retardation assays revealed that the regulatory region located between positions -314 and -135 is a major site of interaction for highly inducible and constitutive nuclear proteins in acute-phase rabbit liver. DNase I footprint and competition analyses showed that this region contains two adjacent nuclear protein binding sites (between -191 and -140) with varying affinity for protein binding. Both of these binding sites are capable of driving cytokine-induced transcription of a reporter gene containing a minimal promoter. Detailed analyses of the inducible nuclear proteins that bind to this promoter element showed that they are homologues of the CCAAT/enhancer binding protein (C/EBP) family. Accumulation of the inducible nuclear factors under acute conditions, when maximal transcription activity has been reported, suggests a critical role for these proteins in the expression of the SAA gene.
A Ray; B K Ray
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Gene expression     Volume:  3     ISSN:  1052-2166     ISO Abbreviation:  Gene Expr.     Publication Date:  1993  
Date Detail:
Created Date:  1994-01-31     Completed Date:  1994-01-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9200651     Medline TA:  Gene Expr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  151-62     Citation Subset:  IM    
Department of Veterinary Microbiology, University of Missouri-Columbia 65211.
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MeSH Terms
Base Sequence
Binding Sites
CCAAT-Enhancer-Binding Proteins
Cloning, Molecular
DNA-Binding Proteins / metabolism*
Deoxyribonuclease I
Gene Expression Regulation
Hot Temperature
Liver / metabolism*
Molecular Sequence Data
Nuclear Proteins / metabolism*
Promoter Regions, Genetic*
Serum Amyloid A Protein / genetics*
Transcription Factors / metabolism
Grant Support
Reg. No./Substance:
0/CCAAT-Enhancer-Binding Proteins; 0/DNA-Binding Proteins; 0/Nuclear Proteins; 0/Serum Amyloid A Protein; 0/Transcription Factors; 9007-49-2/DNA; EC I

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