| Analysis of post-lysosomal compartments. | |
| | |
MedLine Citation:
|
PMID: 14733906 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Lysosomes are acidic intracellular compartments and are regarded as degradative and the end point, of the endocytic pathway. Here we provide evidence for the generation of acid hydrolase poor and non-acidic post-lysosomal compartments in NRK cells that have accumulated non-digestible macromolecules, Texas red-dextran (TR-Dex), within lysosomes. When TR-Dex was fed to the cells for 6h, most of the internalized TR-Dex colocalized with a lysosomal enzyme, cathepsin D. With an increase in the chase period, however, the internalized TR-Dex gradually accumulated in cathepsin D-negative vesicles. These vesicles were positive for a lysosomal membrane protein, LGP85, and their formation was inhibited by treatment of the cells with U18666A, which impairs membrane transport out of late endosomal/lysosomal compartments, thereby suggesting that the vesicles are derived from lysosomes. Interestingly, these compartments are non-acidic as judged for the DAMP staining. The results, therefore, suggest that the excess accumulation of non-digestible macromolecules within lysosomes induces the formation of acid hydrolase poor and non-acidic post-lysosomal compartments. The fact that treatment of the cells with lysosomotropic amines or a microtubule-depolymerization agent resulted in extensive colocalization of TR-Dex with cathepsin D further indicates that the formation of the post-lysosomal compartments depends on the lysosomal acidification and microtubule organization. Furthermore, these results suggest bi-directional membrane transport between lysosomes and the post-lysosomal compartments, which implies that the latter are not resting compartments. |
| | |
Authors:
|
Yuko Hirota; Naoko Masuyama; Toshio Kuronita; Hideaki Fujita; Masaru Himeno; Yoshitaka Tanaka |
Related Documents
:
|
3569636 - Alkaline phosphatase expression in human chorionic villi. 8920106 - Protein phosphatase activity is required for prothoracicotropic hormone-stimulated ecdy... 8978956 - Effects of orally administered bisphosphonates on bone loss in a disuse osteopenia mode... 2565326 - Study of the accuracy of physical and biochemical markers in semen to detect infectious... 1716826 - Acid blockade by omeprazole or ici 162846 in a chronic duodenal ulcer model. 7654776 - Platelet-activating factor and its structural analogues in the earthworm eisenia foetida. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 314 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2004 Feb |
Date Detail:
|
Created Date: 2004-01-21 Completed Date: 2004-03-05 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
|
Languages: eng Pagination: 306-12 Citation Subset: IM |
Affiliation:
|
Division of Pharmaceutical Cell Biology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, 812-8582 Fukuoka, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Androstenes
/
pharmacology Animals Antigens, CD36 / biosynthesis Cathepsin D / biosynthesis, chemistry Dextrans / chemistry Endosomes / metabolism Enzyme Inhibitors / pharmacology Fluorescent Dyes / pharmacology Hydrogen-Ion Concentration Hydrolases / metabolism Kidney Lysosomal Storage Diseases / metabolism Lysosome-Associated Membrane Glycoproteins Lysosomes / metabolism*, physiology Membrane Glycoproteins* Microscopy, Fluorescence Microtubules / metabolism Rats Time Factors Xanthenes / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Androstenes; 0/Antigens, CD36; 0/Enzyme Inhibitors; 0/Fluorescent Dyes; 0/Lysosome-Associated Membrane Glycoproteins; 0/Membrane Glycoproteins; 0/Xanthenes; 139638-11-2/Cd36l2 protein, rat; 3039-71-2/3-beta-(2-(diethylamino)ethoxy)androst-5-en-17-one; 82354-19-6/Texas red; 9004-54-0/Dextrans; EC 3.-/Hydrolases; EC 3.4.23.5/Cathepsin D |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Na(+)-D-glucose cotransporter in muscle capillaries increases glucose permeability.
Next Document: A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell ca...