Document Detail


Analysis of polymorphisms in the promoter region of interleukin-1beta by restriction fragment length polymorphism-PCR.
MedLine Citation:
PMID:  15363219     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the frequencies of -1470, -511 and -31 single nucleotide polymorphisms (SNPs) in the promoter of IL-1beta and its haplotype constitution in Chongqing population. METHODS: One hundred and twelve healthy Chongqing people were enrolled in this study. Polymorphisms at -1470 (G to C), -511 (T to C) and -31 (C to T) of IL-1beta were genotyped with the method of restriction fragment length polymorphism (RFLP). Haplotype frequencies were analyzed by Arlequine software. RESULTS: Frequencies of IL-1beta -1470, -511 and -31 SNPs were 41.67%, 50% and 45.33%, respectively. Genotype frequencies of -1470 locus were 39.81%, 37.04% and 23.15% for G/G, G/C and C/C respectively. As for T-511C SNP, genotype frequencies of T/T, T/C and C/C were 29.91%, 40.18% and 29.91%, respectively. Genotyping results of C/C, C/T, and T/T of -31 locus were 35.51%, 38.32% and 26.71% respectively. Haplotype analysis found that there were mainly three haplotypes constituted by three SNPs, ie., G-T-C, C-T-C and G-C-T. CONCLUSIONS: Polymorphisms exist in the promoter of IL-1beta in Chongqing population. Three SNPs locate in the same haplotype block.
Authors:
Ai-Qing Wen; Jun Wang; Kai Feng; Pei-Fang Zhu; Jian-Xin Jiang
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chinese journal of traumatology = Zhonghua chuang shang za zhi / Chinese Medical Association     Volume:  7     ISSN:  1008-1275     ISO Abbreviation:  Chin. J. Traumatol.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-09-14     Completed Date:  2004-12-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100886162     Medline TA:  Chin J Traumatol     Country:  China    
Other Details:
Languages:  eng     Pagination:  271-4     Citation Subset:  IM    
Affiliation:
Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
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MeSH Terms
Descriptor/Qualifier:
Asian Continental Ancestry Group / genetics*
China
Cohort Studies
Female
Genetics, Population*
Genotype
Haplotypes
Humans
Interleukin-1 / genetics*
Male
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single-Stranded Conformational*
Promoter Regions, Genetic
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Interleukin-1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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