Document Detail


Analysis of plasma ghrelin in patients with medium-chain acyl-CoA dehydrogenase deficiency and glutaric aciduria type II.
MedLine Citation:
PMID:  22048973     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Objective: Ghrelin requires a fatty acid modification for binding to the growth hormone secretagogue receptor. Acylation of the Ser3 residue of ghrelin is essential for its biological activities. We hypothesized that acyl-CoA is the fatty acid substrate for ghrelin acylation. Because serum octanoyl-CoA levels are altered by fatty acid oxidation disorders, we examined circulating ghrelin levels in affected patients.Materials and Methods: Blood levels of acyl (A) and desacyl (D) forms of ghrelin and acylcarnitine of patients with medium chain acyl-CoA dehydrogenase (MCAD) deficiency and glutaric aciduria type II (GA2) were measured.Results: Plasma acyl ghrelin levels and A/D ratios appeared to be elevated in patients with MCAD deficiency or GA2 than in comparison to those in normal subjects. Reverse-phase high-performance liquid chromatography confirmed that n-octanoylated ghrelin levels were elevated in these patients.Conclusion: Changing serum medium-chain acylcarnitine levels may affect circulating acyl ghrelin levels, suggesting that acyl-CoA is the substrate for ghrelin acylation.
Authors:
Takakshi Akamizu; Nobuo Sakura; Yosuke Shigematsu; Go Tajima; Akira Ohtake; Hiroshi Hosoda; Hiroshi Iwakura; Hiroyuki Ariyasu; Kenji Kangawa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-2
Journal Detail:
Title:  European journal of endocrinology / European Federation of Endocrine Societies     Volume:  -     ISSN:  1479-683X     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423848     Medline TA:  Eur J Endocrinol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
T Akamizu, The First Department of Medicine, Wakayama Medical University, Wakayama, Japan.
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