Document Detail

Analysis of plant complex matrices by use of nuclear magnetic resonance spectroscopy: St. John's wort extract.
MedLine Citation:
PMID:  11368564     Owner:  NLM     Status:  MEDLINE    
The efficiency of two-dimensional homonuclear (1)H--(1)H correlated spectroscopy and two-dimensional reverse heteronuclear shift correlation spectroscopy (i.e., heteronuclear multiple quantum correlation) in characterizing and evaluating the relative content of herbal extract constituents is demonstrated. These experiments are able to fully assign the proton and carbon resonances of all three classes of constituents present in dried commercial extract of St. John's wort, that is, flavonols, phloroglucinols, and naphthodianthrones, with particular regard to the very unstable phloroglucinols. In addition, shikimic and chlorogenic acids, sucrose, lipids, polyphenols, and traces of solvents of the extractive process (methanol) were also identified. These experiments can be considered to be a very simple and fast analytical method for determining the quality and stability of the titled commercial extract. They represent a generally applicable technique for a rapid screening and a specific measurement of other commercial phytochemicals or, in selected cases, an alternative to the classical analytical techniques such as high-performance thin-layer chromatography, high-performance liquid chromatography, capillary gas chromatography, and electrophoresis.
A R Bilia; M C Bergonzi; G Mazzi; F F Vincieri
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  49     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-22     Completed Date:  2001-12-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2115-24     Citation Subset:  IM    
Dipartimento di Scienze Farmaceutiche, via Gino Capponi 9, 50121 Florence, Italy.
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MeSH Terms
Drug Stability
Hypericum / chemistry*
Magnetic Resonance Spectroscopy / methods*
Plant Extracts / analysis*
Plants, Medicinal*
Reg. No./Substance:
0/Plant Extracts

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