Document Detail


Analysis of the phylogenetic diversity of estrone-degrading bacteria in activated sewage sludge using microautoradiography-fluorescence in situ hybridization.
MedLine Citation:
PMID:  18513907     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In situ uptake of [2,4,6,7-3H(N)]estrone ([3H]E1) by the major phylogenetic groups present in activated sludge samples from two different municipal wastewater treatment plants was investigated using microautoradiography-fluorescence in situ hybridization (MAR-FISH). Approximately 1-2% of the total cells confined in the samples by an EUB probe mix contributed to E1 assimilation. Almost all the detected E1-assimilating cells involved in the early phase of E1 degradation were affiliated with the Beta- and Gammaproteobacteria. In the early phase of E1 degradation, no E1-assimilating cells affiliated with the Alphaproteobacteria, Actinobacteria, the Cytophaga-Flavobacterium cluster of phylum Bacteroidetes, or the phyla Chloroflexi, Nitrospira and Planctomycetes were detected. Bacteria affiliated with the Betaproteobacteria in the shape of long rods or chains of rods were found to contribute most to in situ E1 degradation. They contributed 61% and 82% of total E1-assimilating cells in cultures from two sources of activated sludge spiked with [3H]E1. The E1-degrading bacteria related to the Betaproteobacteria differed phylogenetically from the aerobic E1-degrading bacterial isolates reported in previous studies. In addition, MAR-FISH revealed the significant contribution of E1-degrading bacteria affiliated with the Gammaproteobacteria in the degradation of E1 in activated sludge.
Authors:
Kaisai Zang; Futoshi Kurisu; Ikuro Kasuga; Hiroaki Furumai; Osami Yagi
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Publication Detail:
Type:  Journal Article     Date:  2008-06-02
Journal Detail:
Title:  Systematic and applied microbiology     Volume:  31     ISSN:  0723-2020     ISO Abbreviation:  Syst. Appl. Microbiol.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-18     Completed Date:  2008-09-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306133     Medline TA:  Syst Appl Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  206-14     Citation Subset:  IM    
Affiliation:
Department of Urban Engineering, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan. kszang@env.t.u-tokyo.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Autoradiography / methods*
Bacteria / classification*,  genetics,  metabolism
Betaproteobacteria / classification,  genetics,  isolation & purification,  metabolism
Biodiversity
DNA, Ribosomal / genetics
Estrone / metabolism*
Gammaproteobacteria / classification,  genetics,  isolation & purification,  metabolism
In Situ Hybridization, Fluorescence / methods*
Oligonucleotide Probes
Phylogeny*
RNA, Ribosomal, 16S / genetics
RNA, Ribosomal, 23S / genetics
Sewage / microbiology*
Waste Disposal, Fluid / methods
Chemical
Reg. No./Substance:
0/DNA, Ribosomal; 0/Oligonucleotide Probes; 0/RNA, Ribosomal, 16S; 0/RNA, Ribosomal, 23S; 0/Sewage; 53-16-7/Estrone

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