| Analysis of photochemically induced cotton bound residues of deltamethrin by an immunoassay technique. | |
| | |
MedLine Citation:
|
PMID: 20166600 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A sensitive and selective indirect competitive ELISA was developed for the detection of deltamethrin bound residues on cotton texture. Cross-reactivity studies with the main deltamethrin photodegradation products showed high specificity of deltamethrin polyclonal antibody to the parent compound. No cross-reactivity was measured with deltamethrin photodegradtion products derived from the alcohol moiety (3-phenoxybenzaldhyde, phenoxybenzyl alcohol, cyanohydrin, and 3-phenoxybenzoic acid), and lesser amounts were observed with the acid moiety (deltamethric acid). The dot-blot immunoassay was performed on cotton fabric discs spiked with deltamethrin and irradiated to assess the suitability of this system to detect bound residue. The dot-blot immunoassay results revealed that the bound form of deltamethrin has binding affinity with deltamethrin antibody similar to the parent compound. In addition, the test system was used to detect bound and free residues of deltamethrin on cotton samples exposed to three cycles of simulated sunlight and water wash. The results obtained suggest that the competitive ELISA format can be used as a tool for monitoring free and bound residues of deltamethrin impregnated on cotton targets. |
| | |
Authors:
|
Hamdy R Soltan; Fawkia A Abd El-Megeed; Fathia I Mustafa; Hanafy M Ismail |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of AOAC International Volume: 92 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2009 Nov-Dec |
Date Detail:
|
Created Date: 2010-02-19 Completed Date: 2010-03-22 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: United States |
Other Details:
|
Languages: eng Pagination: 1815-20 Citation Subset: IM |
Affiliation:
|
Alexandria University, Faculty of Agriculture, Pesticide Chemistry Department, PO Box 21545, Alexandria, Egypt. hrsoltan@hotmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antibody Specificity Cotton Fiber* Cross Reactions Enzyme-Linked Immunosorbent Assay Insecticides / analysis* Laundering Nitriles / analysis* Pesticide Residues / analysis* Photochemistry Pyrethrins / analysis* Reference Standards Textiles / analysis*, radiation effects Ultraviolet Rays Water / analysis |
| Chemical | |
Reg. No./Substance:
|
0/Insecticides; 0/Nitriles; 0/Pesticide Residues; 0/Pyrethrins; 52820-00-5/decamethrin; 7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Simultaneous spectrophotometric determination of benzoic acid, sorbic acid, and ascorbic acid using ...
Next Document: Toward a quality guide to facilitate the transference of analytical methods from research to testing...