Document Detail


Analysis of photochemically induced cotton bound residues of deltamethrin by an immunoassay technique.
MedLine Citation:
PMID:  20166600     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A sensitive and selective indirect competitive ELISA was developed for the detection of deltamethrin bound residues on cotton texture. Cross-reactivity studies with the main deltamethrin photodegradation products showed high specificity of deltamethrin polyclonal antibody to the parent compound. No cross-reactivity was measured with deltamethrin photodegradtion products derived from the alcohol moiety (3-phenoxybenzaldhyde, phenoxybenzyl alcohol, cyanohydrin, and 3-phenoxybenzoic acid), and lesser amounts were observed with the acid moiety (deltamethric acid). The dot-blot immunoassay was performed on cotton fabric discs spiked with deltamethrin and irradiated to assess the suitability of this system to detect bound residue. The dot-blot immunoassay results revealed that the bound form of deltamethrin has binding affinity with deltamethrin antibody similar to the parent compound. In addition, the test system was used to detect bound and free residues of deltamethrin on cotton samples exposed to three cycles of simulated sunlight and water wash. The results obtained suggest that the competitive ELISA format can be used as a tool for monitoring free and bound residues of deltamethrin impregnated on cotton targets.
Authors:
Hamdy R Soltan; Fawkia A Abd El-Megeed; Fathia I Mustafa; Hanafy M Ismail
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  92     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2009 Nov-Dec
Date Detail:
Created Date:  2010-02-19     Completed Date:  2010-03-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1815-20     Citation Subset:  IM    
Affiliation:
Alexandria University, Faculty of Agriculture, Pesticide Chemistry Department, PO Box 21545, Alexandria, Egypt. hrsoltan@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Antibody Specificity
Cotton Fiber*
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Insecticides / analysis*
Laundering
Nitriles / analysis*
Pesticide Residues / analysis*
Photochemistry
Pyrethrins / analysis*
Reference Standards
Textiles / analysis*,  radiation effects
Ultraviolet Rays
Water / analysis
Chemical
Reg. No./Substance:
0/Insecticides; 0/Nitriles; 0/Pesticide Residues; 0/Pyrethrins; 52820-00-5/decamethrin; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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