| Analysis of phenolic glycosides and saponins in Primula elatior and Primula veris (primula root) by liquid chromatography, evaporative light scattering detection and mass spectrometry. | |
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MedLine Citation:
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PMID: 16297921 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This paper describes the first liquid chromatographic method suitable for the simultaneous determination of bioactive compounds, saponins and phenolic glycosides, present in Primula elatior and Primula veris, including the NMR data of primulaverin and primeverin. Optimum separations were obtained with a Synergi 4mum Fusion RP 80A column, using 0.025% TFA in water and 5% acetonitrile in methanol as mobile phase. Saponins were detected by evaporative light scattering detection (ELSD), whereas the phenolic glycosides were monitored by UV at 210 nm. The method was validated for repeatability (sigma(rel) <or=4.5%), precision (intra- and inter-day variation <or=5.0%), accuracy (recovery >or=97.1%) and sensitivity (LOD <or=22 ng (UV) and <or=38 ng (ELSD) on-column, respectively). LC-MS experiments in negative APCI mode allowed a final peak assignment. Both Primula species could easily be differentiated by their saponin pattern. The total saponin content was highest in P. veris roots (max. 14.9%), the aerial parts or P. elatior contained significantly less amounts; primeverin (0.64-1.42%) showed to be the most dominant phenolic glycoside. |
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Authors:
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Andrea Müller; Markus Ganzera; Hermann Stuppner |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies Date: 2005-11-17 |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1112 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-04-17 Completed Date: 2006-06-27 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 218-23 Citation Subset: IM |
Affiliation:
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Institute of Pharmacy, University of Innsbruck, Innrain 52, 6020 Innsbruck, Austria. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Liquid
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methods* Flowers / chemistry Glycosides / analysis* Light Mass Spectrometry / methods* Nuclear Magnetic Resonance, Biomolecular Plant Leaves / chemistry Plant Roots / chemistry Primula / chemistry* Saponins / analysis* Scattering, Radiation |
| Chemical | |
Reg. No./Substance:
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0/Glycosides; 0/Saponins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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