Document Detail


Analysis of phenolic acids by non-aqueous capillary electrophoresis after electrokinetic supercharging.
MedLine Citation:
PMID:  20934181     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Electrokinetic supercharging (EKS), a new and powerful on-line preconcentration method for capillary electrophoresis, was utilized in non-aqueous capillary electrophoresis (NACE) to enhance the sensitivity of phenolic acids. The buffer acidity and concentration, leader and terminator length and electrokinetic injection time were optimised, with the optimum conditions being: a background electrolyte of 40 mM Tris-acetic acid (pH 7.9), hydrodynamic injection of 50 mM ammonium chloride (22 s, 0.5 psi) as leader, electrokinetic injection of the sample (180 s, -10 kV), hydrodynamic injection of 20 mM CHES (32 s, 0.5 psi) as terminator, before application of the separation voltage (-25 kV). Under these conditions the sensitivity was enhanced between 1333 and 3440 times when compared to a normal hydrodynamic injection with the sample volume <3% of the capillary volume. Detection limits for the seven phenolic acids were in the range of 0.22-0.51 ng/mL and EKS was found to be 3.6-7.9 times more sensitive than large-volume sample stacking and anion selective exhaustive injection for the same seven phenolic acids.
Authors:
Yuanqi Lu; Michael C Breadmore
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-08
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1217     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-25     Completed Date:  2011-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  7282-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Affiliation:
Australian Centre for Research on Separation Science, School of Chemistry, University of Tasmania, Hobart, Tasmania 7001, Australia.
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MeSH Terms
Descriptor/Qualifier:
Chemical Fractionation
Electrophoresis, Capillary / methods*
Hydrogen-Ion Concentration
Hydroxybenzoic Acids / analysis*,  isolation & purification
Regression Analysis
Sensitivity and Specificity
Time Factors
Chemical
Reg. No./Substance:
0/Hydroxybenzoic Acids; 29656-58-4/phenolic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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