| Analysis of phenolic acids by non-aqueous capillary electrophoresis after electrokinetic supercharging. | |
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MedLine Citation:
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PMID: 20934181 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Electrokinetic supercharging (EKS), a new and powerful on-line preconcentration method for capillary electrophoresis, was utilized in non-aqueous capillary electrophoresis (NACE) to enhance the sensitivity of phenolic acids. The buffer acidity and concentration, leader and terminator length and electrokinetic injection time were optimised, with the optimum conditions being: a background electrolyte of 40 mM Tris-acetic acid (pH 7.9), hydrodynamic injection of 50 mM ammonium chloride (22 s, 0.5 psi) as leader, electrokinetic injection of the sample (180 s, -10 kV), hydrodynamic injection of 20 mM CHES (32 s, 0.5 psi) as terminator, before application of the separation voltage (-25 kV). Under these conditions the sensitivity was enhanced between 1333 and 3440 times when compared to a normal hydrodynamic injection with the sample volume <3% of the capillary volume. Detection limits for the seven phenolic acids were in the range of 0.22-0.51 ng/mL and EKS was found to be 3.6-7.9 times more sensitive than large-volume sample stacking and anion selective exhaustive injection for the same seven phenolic acids. |
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Authors:
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Yuanqi Lu; Michael C Breadmore |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-08 |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1217 ISSN: 1873-3778 ISO Abbreviation: J Chromatogr A Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-25 Completed Date: 2011-01-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 7282-7 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Australian Centre for Research on Separation Science, School of Chemistry, University of Tasmania, Hobart, Tasmania 7001, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chemical Fractionation Electrophoresis, Capillary / methods* Hydrogen-Ion Concentration Hydroxybenzoic Acids / analysis*, isolation & purification Regression Analysis Sensitivity and Specificity Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Hydroxybenzoic Acids; 29656-58-4/phenolic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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