Document Detail

Analysis of peptides from pigeon cerebrum: a comparison of sacrifice methods.
MedLine Citation:
PMID:  318188     Owner:  NLM     Status:  MEDLINE    
Peptides were extracted and fractionated from pigeon cerebrum and analyzed by thin-layer chromatography, polyacrylamide disc gel electrophoresis, and amino acid analysis. A highly consistent pattern of peptides in the 700-5000 molecular weight range was obtained from each cerebral sample. A comparison was also made between cerebral peptide patterns obtained from animals sacrificed by decapitation with those from animals sacrificed by microwave irradiation. Amino acid analysis of the peptide fraction from microwaved and decapitated animals revealed slight differences in the amount and incidence of various amino acids. These differences may be due to post-mortem proteolysis in the cerebra of decapitated pigeons prior to homogenization and freezing.
R A Barraco; M S Abel; D M Terrian
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research bulletin     Volume:  3     ISSN:  0361-9230     ISO Abbreviation:  Brain Res. Bull.     Publication Date:    1978 Nov-Dec
Date Detail:
Created Date:  1989-03-22     Completed Date:  1989-03-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7605818     Medline TA:  Brain Res Bull     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  645-53     Citation Subset:  IM    
Department of Physiology, Wayne State University, School of Medicine, Detroit, MI 48201.
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MeSH Terms
Amino Acids / analysis
Brain / radiation effects
Brain Chemistry*
Columbidae / metabolism*
Peptides / analysis*
Reg. No./Substance:
0/Amino Acids; 0/Peptides

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