Document Detail


Analysis of pentacyclic triterpenes by LC-MS. A comparative study between APCI and APPI.
MedLine Citation:
PMID:  18946879     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The analytical performances of three atmospheric-pressure sources, electrospray (ESI), atmospheric-pressure chemical ionization (APCI), and atmospheric-pressure photoionization (APPI), were evaluated for the analysis of pentacyclic triterpenes in liquid chromatography-mass spectrometry (LC-MS). Among these sources, APPI and APCI are particularly well adapted to sensitive analyses of pentacyclic triterpenes by LC-MS. Detection parameters were optimized for both the sources, and the effects of three dopants (toluene, acetone and anisole) on the detection (sensitivity and ion fingerprints in MS spectra) were studied in detail for APPI-MS.The limits of quantification were measured under selected ion monitoring conditions, in the range of 0.005-0.015 mg l(-1) and 0.002-0.84 mg l(-1) in APPI and APCI, respectively, depending on the studied pentacyclic triterpene. Overall, APPI was found more sensitive than APCI in positive ion mode, whereas APCI shows the greatest sensitivity for acidic triterpenes in negative ion mode.Following this study, the developed LC-MS method was used for the characterization of pentacyclic triterpenes in three plant extracts. High amounts of betulinic acid, betulinic aldehyde and betulinic aldehyde acetate were observed in plane bark. The main component of birch bark is betulin and extracts of okoume resin exhibit high amounts of alpha- and beta-amyrin.
Authors:
B Rhourri-Frih; P Chaimbault; B Claude; C Lamy; P André; M Lafosse
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of mass spectrometry : JMS     Volume:  44     ISSN:  1076-5174     ISO Abbreviation:  J Mass Spectrom     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-13     Completed Date:  2009-03-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9504818     Medline TA:  J Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  71-80     Citation Subset:  IM    
Copyright Information:
(c) 2008 John Wiley & Sons, Ltd.
Affiliation:
Institut de Chimie Organique et Analytique (ICOA), UMR-CNRS 6005, Université d'Orléans, Orléans Cedex 2, France.
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MeSH Terms
Descriptor/Qualifier:
Acetone / chemistry
Anisoles / chemistry
Atmospheric Pressure
Chromatography, Liquid / methods*
Mass Spectrometry / methods*
Pentacyclic Triterpenes / analysis*
Plant Extracts / analysis
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization / methods
Toluene / chemistry
Chemical
Reg. No./Substance:
0/Anisoles; 0/Pentacyclic Triterpenes; 0/Plant Extracts; 100-66-3/anisole; 108-88-3/Toluene; 67-64-1/Acetone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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