Document Detail


Analysis of pathogenicity by restriction-endonuclease digestion of amplified genomic DNA of Entamoeba histolytica isolated in Pernambuco, Brazil.
MedLine Citation:
PMID:  1495923     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The pathogenicity of 47 strains of Entamoeba histolytica isolated in Pernambuco, Brazil, was examined using the polymerase chain reaction (PCR) followed by restriction-endonuclease digestion. Electrophoretic patterns of PCR products digested with HinfI revealed that all strains were nonpathogenic. The results were entirely in accord with phenotypic properties such as isoenzyme patterns and the failure to bind a pathogenic-isolate-specific monoclonal antibody. When the sensitivity of PCR was examined, amplified products could be detected from template DNA equivalent to five trophozoites. These observations indicate that PCR amplification of genomic DNA and subsequent restriction-enzyme digestion is a useful strategy for obtaining a sensitive and accurate diagnosis. The present study also demonstrates that nonpathogenic strains of E. histolytica predominate in northeastern Brazil.
Authors:
H Tachibana; S Kobayashi; K C Paz; I S Aca; S Tateno; S Ihara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Parasitology research     Volume:  78     ISSN:  0932-0113     ISO Abbreviation:  Parasitol. Res.     Publication Date:  1992  
Date Detail:
Created Date:  1992-09-04     Completed Date:  1992-09-04     Revised Date:  2008-08-29    
Medline Journal Info:
Nlm Unique ID:  8703571     Medline TA:  Parasitol Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  433-6     Citation Subset:  IM    
Affiliation:
Laboratório de Imunopatologia Prof. Keizo Asami, Universidade Federal de Pernambuco, Recife, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Brazil
DNA, Protozoan / analysis*
Deoxyribonucleases, Type II Site-Specific
Entamoeba histolytica / classification,  enzymology,  genetics,  pathogenicity*
Entamoebiasis / parasitology*
Humans
Isoenzymes / analysis
Molecular Sequence Data
Polymerase Chain Reaction
Restriction Mapping
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/DNA, Protozoan; 0/Isoenzymes; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific; EC 3.1.21.4/GANTC-specific type II deoxyribonucleases

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