Document Detail


Analysis of papillomavirus consensus L1 gene in a closed colony of baboons (Papio anubis).
MedLine Citation:
PMID:  10819813     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The purpose of this study was to analyze cervical specimens and semen from a closed colony of baboons for the presence of the papillomavirus consensus L1 gene.
STUDY DESIGN: Cervical swabs were collected from lightly anesthetized female baboons. Semen was collected from a male baboon by standard electroejaculation techniques. Deoxyribonucleic acid was extracted from the cells by two different methods and analyzed by polymerase chain reaction targeting the L1 consensus gene common for >25 genital papillomaviruses.
RESULTS: Analyses of the polymerase chain reaction-amplified products did not reveal bands for the papillomavirus in either the cervical specimens or the semen.
CONCLUSIONS: The hypothesis of a linkage between primates with papillomavirus as a common factor is not supported by the results of this study. This information is also important in assisting clinicians in setting up specific pathogen-free colonies of baboons.
Authors:
D E Wonderly; P J Chan; S Cseh; J D Jacobson; L Bailey
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  182     ISSN:  0002-9378     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-06-15     Completed Date:  2000-06-15     Revised Date:  2014-07-28    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1016-7     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Capsid Proteins
Cervix Uteri / virology
Consensus Sequence*
DNA, Viral / analysis
Female
Genes, Viral*
Male
Oncogene Proteins, Viral / genetics*
Papillomaviridae / genetics*
Papio / virology*
Polymerase Chain Reaction
Semen / virology
Chemical
Reg. No./Substance:
0/Capsid Proteins; 0/DNA, Viral; 0/HPV L1 protein, Human papillomavirus; 0/Oncogene Proteins, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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