Document Detail

Analysis of pancreatic islet cells and hormone content in the spontaneously diabetic KKAy mouse by morphometry, immunocytochemistry and radioimmunoassay.
MedLine Citation:
PMID:  2446417     Owner:  NLM     Status:  MEDLINE    
The splenic pancreas of 165 day old diabetic KKAy and age-matched nondiabetic C57BL/6 mice was examined by morphometry and immunocytochemistry at the light microscopic level and by radioimmunoassay to evaluate the morphology, surface area, endocrine cell composition and hormone content of the pancreatic islets. The insulin cells of the diabetic mice were severely degranulated and many of the glucagon, somatostatin and pancreatic polypeptide cells were displaced from the mantle to the core of the islet tissue where the non-insulin cells appeared to lose their continuity. The topography of some of the islets of KKAy mice was further deranged by acinar cells among the endocrine tissue. Morphometric analysis revealed that the surface area of the islets of KKAy mice was significantly expanded in comparison with that of C57BL/6 mice. The volume and numerical percents of the insulin cells were significantly increased whereas those of the glucagon and somatostatin cells were decreased in the KKAy mice. Since only the mean absolute number of insulin cells was elevated in the diabetic mice, the alteration in the relative proportions of the non-insulin cells and hypertrophy of the islets seemed to be a manifestation of insulin cell hyperplasia. Pancreatic insulin and somatostatin contents were markedly diminished in the islets of KKAy compared with those of C57BL/6 mice. These results demonstrate that the microscopic anatomy, endocrine cell populations and hormone content of the pancreatic islets are deranged in the KKAy mouse with severe hyperinsulinemia and hyperglycemia.
A R Diani; G A Sawada; B A Hannah; K S Jodelis; M A Connell; C L Connell; T J Vidmar; B M Wyse
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Virchows Archiv. A, Pathological anatomy and histopathology     Volume:  412     ISSN:  0174-7398     ISO Abbreviation:  Virchows Arch A Pathol Anat Histopathol     Publication Date:  1987  
Date Detail:
Created Date:  1988-01-13     Completed Date:  1988-01-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8302198     Medline TA:  Virchows Arch A Pathol Anat Histopathol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  53-61     Citation Subset:  IM    
Diabetic and Gastrointestinal Diseases Research, Upjohn Company, Kalamazoo, MI 49001.
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MeSH Terms
Blood Glucose / analysis
Diabetes Mellitus, Experimental / metabolism,  pathology*
Glucagon / analysis
Hemoglobin A, Glycosylated / analysis
Insulin / analysis
Islets of Langerhans / metabolism,  pathology*
Mice, Inbred C57BL
Mice, Obese / metabolism*
Pancreatic Hormones / analysis,  metabolism*
Pancreatic Polypeptide / analysis
Somatostatin / analysis
Staining and Labeling / methods
Reg. No./Substance:
0/Blood Glucose; 0/Hemoglobin A, Glycosylated; 0/Pancreatic Hormones; 11061-68-0/Insulin; 51110-01-1/Somatostatin; 59763-91-6/Pancreatic Polypeptide; 9007-92-5/Glucagon

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