Document Detail


Analysis of oxazepam in urine using solid-phase extraction and high-performance liquid chromatography with fluorescence detection by post-column derivatization.
MedLine Citation:
PMID:  8376518     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A reversed-phase high-performance liquid chromatographic method for oxazepam in human urine samples has been developed. The sample preparation consists of an enzymatic hydrolysis with beta-glucuronidase, followed by a solid-phase extraction process using Bond-Elut C2 cartridges. The mobile phase used was a methanol-water (60:40, v/v) mixture at a flow-rate of 0.50 ml/min. The column was a 3.5 cm x 4.6 mm I.D. C18 reversed-phase column. The detection system was based on a fluorescence post-column derivatization of oxazepam in mixtures of methanol and acetic acid. A linear range from 0.01 to 1 micrograms/ml of urine and a limit of detection of 4 ng/ml of urine were attained. Within-day recoveries and reproducibilities from urine samples spiked with 0.2 and 0.02 microgram/ml oxazepam were 97.9 and 95.0 and 2.1 and 9.4%, respectively.
Authors:
L A Berrueta; B Gallo; F Vicente
Related Documents :
2556418 - Determination of tazobactam and piperacillin in human plasma, serum, bile and urine by ...
8491818 - Determination of methotrexate in human urine at nanomolar levels by high-performance li...
6715468 - Improved method for the determination of chlorhexidine in urine.
25149008 - Ligandless ultrasonic-assisted and ionic liquid-based dispersive liquid-liquid microext...
12051338 - Identification of human urine stains by hplc analysis of 17-ketosteroid conjugates.
11197468 - Did nato attacks in yugoslavia cause a detectable environmental effect in hungary?
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  616     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-10-18     Completed Date:  1993-10-18     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  344-8     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid
Humans
Indicators and Reagents
Nordazepam / urine
Oxazepam / pharmacokinetics,  urine*
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 67220MCM01/Nordazepam; 6GOW6DWN2A/Oxazepam

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  High-performance liquid chromatographic method for the determination of a candidate 8-aminoquinoline...
Next Document:  Determination of tenoxicam in plasma by high-performance liquid chromatography.