Document Detail

Analysis of oxazepam in urine using solid-phase extraction and high-performance liquid chromatography with fluorescence detection by post-column derivatization.
MedLine Citation:
PMID:  8376518     Owner:  NLM     Status:  MEDLINE    
A reversed-phase high-performance liquid chromatographic method for oxazepam in human urine samples has been developed. The sample preparation consists of an enzymatic hydrolysis with beta-glucuronidase, followed by a solid-phase extraction process using Bond-Elut C2 cartridges. The mobile phase used was a methanol-water (60:40, v/v) mixture at a flow-rate of 0.50 ml/min. The column was a 3.5 cm x 4.6 mm I.D. C18 reversed-phase column. The detection system was based on a fluorescence post-column derivatization of oxazepam in mixtures of methanol and acetic acid. A linear range from 0.01 to 1 micrograms/ml of urine and a limit of detection of 4 ng/ml of urine were attained. Within-day recoveries and reproducibilities from urine samples spiked with 0.2 and 0.02 microgram/ml oxazepam were 97.9 and 95.0 and 2.1 and 9.4%, respectively.
L A Berrueta; B Gallo; F Vicente
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  616     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-10-18     Completed Date:  1993-10-18     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  344-8     Citation Subset:  IM    
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MeSH Terms
Chromatography, High Pressure Liquid
Indicators and Reagents
Nordazepam / urine
Oxazepam / pharmacokinetics,  urine*
Spectrometry, Fluorescence
Reg. No./Substance:
0/Indicators and Reagents; 67220MCM01/Nordazepam; 6GOW6DWN2A/Oxazepam

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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