| Analysis of oxazepam in urine using solid-phase extraction and high-performance liquid chromatography with fluorescence detection by post-column derivatization. | |
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MedLine Citation:
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PMID: 8376518 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A reversed-phase high-performance liquid chromatographic method for oxazepam in human urine samples has been developed. The sample preparation consists of an enzymatic hydrolysis with beta-glucuronidase, followed by a solid-phase extraction process using Bond-Elut C2 cartridges. The mobile phase used was a methanol-water (60:40, v/v) mixture at a flow-rate of 0.50 ml/min. The column was a 3.5 cm x 4.6 mm I.D. C18 reversed-phase column. The detection system was based on a fluorescence post-column derivatization of oxazepam in mixtures of methanol and acetic acid. A linear range from 0.01 to 1 micrograms/ml of urine and a limit of detection of 4 ng/ml of urine were attained. Within-day recoveries and reproducibilities from urine samples spiked with 0.2 and 0.02 microgram/ml oxazepam were 97.9 and 95.0 and 2.1 and 9.4%, respectively. |
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Authors:
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L A Berrueta; B Gallo; F Vicente |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography Volume: 616 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1993 Jul |
Date Detail:
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Created Date: 1993-10-18 Completed Date: 1993-10-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 344-8 Citation Subset: IM |
Affiliation:
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Department of Analytical Chemistry, Faculty of Sciences, University of the Basque Country, Bilbao, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, High Pressure Liquid Humans Indicators and Reagents Nordazepam / urine Oxazepam / pharmacokinetics, urine* Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; 1088-11-5/Nordazepam; 604-75-1/Oxazepam |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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