Document Detail


Analysis of an outbreak of Salmonella enteritidis by repetitive-sequence-based PCR and pulsed-field gel electrophoresis.
MedLine Citation:
PMID:  20045998     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The aim of this study was to investigate a large food-borne outbreak associated with eggs contaminated by Salmonella Enteritidis in a military unit using pulse field gel electrophoresis (PFGE) and the Repetitive-sequence-based PCR (rep-PCR) employing the DiversiLab system. MATERIALS AND METHODS: In mid-January 2008, a food-borne outbreak associated with S. Enteritidis occurred in a military unit located in the city centre of Isparta. A total of 2,469 patients were registered to six hospitals with gastrointestinal disease symptoms such as diarrhea and abdominal pain. Of those registered, 445 were hospitalized. S. Enteritidis was isolated from 276 stool samples and a blood sample of the hospitalized patients and from a food item. The PFGE patterns after XbaI digestion and rep-PCR profiles produced by the DiversiLab system were determined for eight randomly selected stool isolates, one blood isolate and one food isolate. RESULTS: The PFGE patterns of all isolates were identical. The Rep-PCR profiles produced by using the DiversiLab system showed that all isolates were indistinguishable. The PFGE and rep-PCR interpretations were concordant for the S. Enteritidis isolates. All stool isolates, one blood isolate and one food isolate were susceptible to all antibiotics tested. CONCLUSION: This data suggest that the DiversiLab system may be a reasonable alternative to PFGE for investigation and control of S. Enteritidis outbreaks, since it is easy to use, rapid and does not require highly skilled operators.
Authors:
Abdullah Kilic; Orhan Bedir; Nafiz Kocak; Belkis Levent; Can Polat Eyigun; Omer Faruk Tekbas; Levent Gorenek; Orhan Baylan; A Celal Basustaoglu
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Publication Detail:
Type:  Journal Article     Date:  2010-01-01
Journal Detail:
Title:  Internal medicine (Tokyo, Japan)     Volume:  49     ISSN:  1349-7235     ISO Abbreviation:  Intern. Med.     Publication Date:  2010  
Date Detail:
Created Date:  2010-01-04     Completed Date:  2010-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9204241     Medline TA:  Intern Med     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  31-6     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, Ankara, Turkey. abkilic@gata.edu.tr
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MeSH Terms
Descriptor/Qualifier:
Adult
Disease Outbreaks*
Eggs / microbiology
Electrophoresis, Gel, Pulsed-Field
Genotype
Humans
Male
Military Personnel*
Polymerase Chain Reaction
Salmonella Food Poisoning / diagnosis,  epidemiology*
Salmonella enteritidis / genetics*,  isolation & purification*
Young Adult

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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