Document Detail


Analysis of opioid and non-opioid end products of pro-dynorphin in the substantia nigra of the rat.
MedLine Citation:
PMID:  2860608     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The substantia nigra is among the richest pro-dynorphin terminal field regions in the rat brain. We therefore contrasted processing in this area to the known processing in the posterior pituitary. Fractionation of acid extracts of the posterior pituitary by gel filtration followed by analysis by radioimmunoassay indicated that the molar ratio of dynorphin A(1-17) to dynorphin A(1-8) averaged 1:2. The levels of dynorphin A-related end products to alpha-neo-endorphin and bridge peptide (a 2K nonopioid end product of pro-dynorphin) were approximately equimolar; however, the levels of dynorphin B-sized material were 50% lower than dynorphin A levels. Similar analyses of acid extracts of the substantia nigra also indicated that the levels of dynorphin A, alpha-neo-endorphin, and bridge peptide were approximately equimolar. In this terminal field the levels of dynorphin B-sized material were approximately 60% lower than dynorphin A. A striking feature of the nigral system was that the molar ratio of dynorphin A(1-17) to dynorphin A(1-8) averaged 1:16. Thus, in the nigra, dynorphin A(1-17) is primarily a biosynthetic intermediate rather than as an end product.
Authors:
R M Dores; M E Lewis; H Khachaturian; S J Watson; H Akil
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neuropeptides     Volume:  5     ISSN:  0143-4179     ISO Abbreviation:  Neuropeptides     Publication Date:  1985 Feb 
Date Detail:
Created Date:  1985-07-09     Completed Date:  1985-07-09     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8103156     Medline TA:  Neuropeptides     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  501-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Dynorphins / analogs & derivatives,  analysis,  metabolism*
Endorphins / analysis
Male
Peptide Fragments / analysis
Protein Precursors / analysis
Rats
Rats, Inbred Strains
Substantia Nigra / analysis,  metabolism*
Chemical
Reg. No./Substance:
0/Endorphins; 0/Peptide Fragments; 0/Protein Precursors; 0/dynorphin bridge peptide; 69671-17-6/alpha-neoendorphin; 74913-18-1/Dynorphins; 75790-53-3/dynorphin (1-8); 83335-41-5/rimorphin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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