Document Detail


Analysis of the nucleolar organizing regions in the ant Tapinoma nigerrimum (Hymenoptera, Formicidae).
MedLine Citation:
PMID:  9203352     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study analyses the NORs of Tapinoma nigerrimum, a species that, as known from previous studies, has various chromosomes which carry a NOR site. The analysis was made by a combination of three methods: silver nitrate staining, in situ hybridization with fluorescein-or digoxigenin-labelled probes, and staining with the CG-specific fluorochrome chromomycin A3. The silver staining technique showed an Ag-positive region on chromosome 6 and on various other chromosomes. However, the application of in situ hybridization techniques showed only one positive signal in the proximal region of the short arm of chromosome 6 of T. nigerrimum. Similar results were observed by CMA banding. The absence of rDNA genes or the presence of only a small number of these, not detectable with the above probes, might explain the absence of hybridization signal in the remaining chromosomes.
Authors:
P Lorite; A E Aránega; F Luque; T Palomeque
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Heredity     Volume:  78 ( Pt 6)     ISSN:  0018-067X     ISO Abbreviation:  Heredity     Publication Date:  1997 Jun 
Date Detail:
Created Date:  1997-08-06     Completed Date:  1997-08-06     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0373007     Medline TA:  Heredity     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  578-82     Citation Subset:  IM    
Affiliation:
Departamento de Biologia Experimental, Universidad de Jaén, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Ants / genetics*
Chromomycin A3 / chemistry
DNA Probes
DNA, Ribosomal / genetics
Digoxigenin / chemistry
Diploidy
Female
Fluorescein
Fluoresceins / chemistry
Genes, Insect
Haploidy
In Situ Hybridization, Fluorescence / methods*
Male
Nucleolus Organizer Region / genetics*
Silver Nitrate / chemistry
Silver Staining
Chemical
Reg. No./Substance:
0/DNA Probes; 0/DNA, Ribosomal; 0/Fluoresceins; 1672-46-4/Digoxigenin; 2321-07-5/Fluorescein; 7059-24-7/Chromomycin A3; 7761-88-8/Silver Nitrate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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