Document Detail

Analysis of nitrite and nitrate in biological fluids by capillary electrophoresis.
MedLine Citation:
PMID:  10074886     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: We report a method for determining nitrite and nitrate in biological fluids by capillary electrophoresis. METHODS: A Waters capillary electrophoresis system was used with a filter for detection at 214 nm. After dilution with distilled water, the sample was loaded hydrostatically onto a 60 cm x 100 microm capillary and electrophoresed at 15 kV in 15 mmol/L sulfate buffer, pH 8.0, containing 2.5% electroosmotic flow modifier. RESULTS: The retention times for nitrite and nitrate were 3.9 +/- 0.8 and 4.0 +/- 0.8 min, respectively. The detection limit was 10 micromol/L for serum nitrate. The recovery was 93-115% for nitrite and 92-106% for nitrate. The within-day and between-day coefficients of variation were lower than 3.3% and 5.0%, respectively, for two pools with normal (28 micromol/L) and high (87 micromol/L) nitrate concentration. A comparison with the nitrate reductase method gave a correlation coefficient of 0.982. CONCLUSION: Capillary electrophoresis provides many advantages, namely low cost, small sample and buffer requirements, rapidity, which makes its use particularly suitable for clinical laboratories.
P N Bories; E Scherman; L Dziedzic
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical biochemistry     Volume:  32     ISSN:  0009-9120     ISO Abbreviation:  Clin. Biochem.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-05-21     Completed Date:  1999-05-21     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  0133660     Medline TA:  Clin Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9-14     Citation Subset:  IM    
Laboratoire de Biochimie, Hôpital Albert Chenevier, Assistance Publique-Hôpitaux de Paris, Créteil, France.
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MeSH Terms
Dose-Response Relationship, Drug
Electrophoresis, Capillary / methods*
Nitrate Reductase
Nitrate Reductases / metabolism
Nitrates / blood*,  urine
Nitrites / blood*,  urine
Time Factors
Urinary Tract Infections / urine
Reg. No./Substance:
0/Nitrates; 0/Nitrites; EC 1.7.-/Nitrate Reductases; EC Reductase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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