| Analysis of monosaccharides, fatty constituents and rare O-acetylated sialic acids from gonads of the starfish Asterias rubens. | |
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MedLine Citation:
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PMID: 16181722 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A previous study (Bergwerff et al., Biochimie 74 (1992) 25-37) reported that sialic acids present in Asterias rubens gonads were essentially composed of 8-methyl-N-glycolylneuraminic acid (Neu5Gc8Me), a large part of it being acetylated in position 9. Using GC/MS of heptafluorobutyrate derivatives (Zanetta et al., Glycobiology 11 (2001) 663-676) on the chloroform/methanol soluble and insoluble fractions, we showed that most sialic acids were found in the latter and demonstrated that all sialic acids were derived from N-glycolylneuraminic acid, most of them being 8-methylated, but that the majority were also acetylated in position 4 or 7 (or both positions). GC/MS analyses of the constituents liberated using acid-catalysed methanolysis verified that major glycoprotein-bound glycans were N-linked and of the gluco-oligomannosidic type. Major fatty acids were poly-unsaturated (especially C20:4) and long-chain bases were C22:1 phytosphingosine and C22:2 6-hydroxysphingenine. Major monosaccharides found in the chloroform/methanol extract (quinovose and fucose) were derived from steroidal saponins. |
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Authors:
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Jean-Pierre Zanetta; Vinayaga Srinivasan; Roland Schauer |
Publication Detail:
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Type: Journal Article Date: 2005-08-19 |
Journal Detail:
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Title: Biochimie Volume: 88 ISSN: 0300-9084 ISO Abbreviation: Biochimie Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2005-11-28 Completed Date: 2006-03-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1264604 Medline TA: Biochimie Country: France |
Other Details:
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Languages: eng Pagination: 171-8 Citation Subset: IM |
Affiliation:
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CNRS Unité Mixte de Recherche 8576, Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille Bâtiment C9, 59655 Villeneuve d'Ascq cedex, France. Jean-Pierre.Zanetta@univ-lille1.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Asterias* / anatomy & histology, chemistry Fatty Acids / analysis* Gonads / chemistry* Molecular Structure Monosaccharides / analysis* Sialic Acids / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Monosaccharides; 0/Sialic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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