Document Detail


Analysis of monosaccharides, fatty constituents and rare O-acetylated sialic acids from gonads of the starfish Asterias rubens.
MedLine Citation:
PMID:  16181722     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A previous study (Bergwerff et al., Biochimie 74 (1992) 25-37) reported that sialic acids present in Asterias rubens gonads were essentially composed of 8-methyl-N-glycolylneuraminic acid (Neu5Gc8Me), a large part of it being acetylated in position 9. Using GC/MS of heptafluorobutyrate derivatives (Zanetta et al., Glycobiology 11 (2001) 663-676) on the chloroform/methanol soluble and insoluble fractions, we showed that most sialic acids were found in the latter and demonstrated that all sialic acids were derived from N-glycolylneuraminic acid, most of them being 8-methylated, but that the majority were also acetylated in position 4 or 7 (or both positions). GC/MS analyses of the constituents liberated using acid-catalysed methanolysis verified that major glycoprotein-bound glycans were N-linked and of the gluco-oligomannosidic type. Major fatty acids were poly-unsaturated (especially C20:4) and long-chain bases were C22:1 phytosphingosine and C22:2 6-hydroxysphingenine. Major monosaccharides found in the chloroform/methanol extract (quinovose and fucose) were derived from steroidal saponins.
Authors:
Jean-Pierre Zanetta; Vinayaga Srinivasan; Roland Schauer
Publication Detail:
Type:  Journal Article     Date:  2005-08-19
Journal Detail:
Title:  Biochimie     Volume:  88     ISSN:  0300-9084     ISO Abbreviation:  Biochimie     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2005-11-28     Completed Date:  2006-03-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  France    
Other Details:
Languages:  eng     Pagination:  171-8     Citation Subset:  IM    
Affiliation:
CNRS Unité Mixte de Recherche 8576, Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille Bâtiment C9, 59655 Villeneuve d'Ascq cedex, France. Jean-Pierre.Zanetta@univ-lille1.fr
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MeSH Terms
Descriptor/Qualifier:
Animals
Asterias* / anatomy & histology,  chemistry
Fatty Acids / analysis*
Gonads / chemistry*
Molecular Structure
Monosaccharides / analysis*
Sialic Acids / analysis*
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Monosaccharides; 0/Sialic Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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