Document Detail


Analysis of the midbrain-hindbrain boundary cell fate using a boundary cell-specific Cre-mouse strain.
MedLine Citation:
PMID:  18196597     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe here a transgenic mouse line MHB-Cre, which expresses Cre recombinase in a group of cells at the midbrain-hindbrain boundary. Using this mouse line, we studied the contribution of the boundary cells to distinct brain areas during development. Initially, the MHB-Cre expression coincides with that of Cdh22 and p21 around the Otx2 expression border in a narrow population of cells with reduced proliferative activity. Consistent with their location on both sides of the Otx2 expression border, the Cre expressing boundary cells contribute both to midbrain as well as hindbrain. However, the majority of recombinant cells remain close to the mid- and hindbrain border, suggesting very limited cell mixing within these brain compartments during development. Interestingly, dorsocaudally oriented fibers of the midbrain dopaminergic neurons follow the path marked by the boundary cells.
Authors:
Kaia Kala; Tomi Jukkola; Illar Pata; Juha Partanen
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Publication Detail:
Type:  Letter; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genesis (New York, N.Y. : 2000)     Volume:  46     ISSN:  1526-968X     ISO Abbreviation:  Genesis     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-21     Completed Date:  2008-04-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100931242     Medline TA:  Genesis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  29-36     Citation Subset:  IM    
Copyright Information:
(c) 2008 Wiley-Liss, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / pathology
Cell Lineage
Cell Proliferation
Dopamine / metabolism
Gene Expression Regulation, Developmental*
Genetic Techniques*
Integrases / metabolism*
Mesencephalon / anatomy & histology*
Mice
Mice, Inbred C57BL
Mice, Transgenic
Neurons / metabolism
Rhombencephalon / anatomy & histology*
Transgenes
Chemical
Reg. No./Substance:
EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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