| Analysis of microRNA expression profiles during the cell cycle in synchronized HeLa cells. | |
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MedLine Citation:
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PMID: 19788861 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell cycle progression is regulated by both transcriptional and post-transcriptional mechanisms. MicroRNAs (miRNAs) emerge as a new class of small non-coding RNA regulators of cell cycle as recent evidence suggests. It is hypothesized that expression of specific miRNAs oscillates orderly along with cell cycle progression. However, the oscillated expression patterns of many candidate miRNAs have yet to be determined. Here, we describe miRNA expression profiling in double-thymidine synchronized HeLa cells as cell cycle progresses. Twenty-five differentially expressed miRNAs were classified into five groups based on their cell cycle-dependent expression patterns. The cyclic expression of six miRNAs (miR-221, let-7a, miR-21, miR-34a, miR-24, miR-376b) was validated by real-time quantitative RT-PCR (qRT-PCR). These results suggest that specific miRNAs, along with other key factors are required for maintaining and regulating proper cell cycle progression. The study deepens our understanding on cell cycle regulation. [BMB reports 2009; 42(9): 593-598]. |
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Authors:
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Jue-Yu Zhou; Wen-Li Ma; Shuang Liang; Ye Zeng; Rong Shi; Hai-Lang Yu; Wei-Wei Xiao; Wen-Ling Zheng |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: BMB reports Volume: 42 ISSN: 1976-6696 ISO Abbreviation: BMB Rep Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-09-30 Completed Date: 2009-11-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101465334 Medline TA: BMB Rep Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 593-8 Citation Subset: IM |
Affiliation:
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Institute of Genetic Engineering, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R. China. zhoujueyu@126.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Markers
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analysis* Cell Cycle* Computational Biology Flow Cytometry Gene Expression Profiling* Hela Cells Humans MicroRNAs / genetics*, metabolism Oligonucleotide Array Sequence Analysis RNA, Messenger / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/MicroRNAs; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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