| Analysis of meloxicam by high-performance liquid chromatography with cloud-point extraction. | |
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MedLine Citation:
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PMID: 18751683 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple cloud-point extraction method for the determination of meloxicam in human serum was developed. Meloxicam was extracted from serum sample after adding 1 mL of 3% (v/v) Triton X-114 aqueous solution in the presence of 1M HCl and 60 mg NaCl. The meloxicam, present in the surfactant-rich phase, was enriched again with acetonitrile. Tenoxicam was used as the external standard. The separation was achieved on a C18 analytical column with a mobile phase consisting of aqueous acetic acid (1%, v/v) and acetonitrile (54:46, v/v). UV detection was performed at 360 nm. The response was linear over the range 45-2000 ng mL(-1) in human serum, and intra- and interday precisions of less than 15.0% were obtained. The relative error was within +/-3.0%. The recoveries of meloxicam were larger than 92.0%. The method was compared with liquid-liquid extraction. The results showed that the new method has a considerable LOQ and higher recoveries but poorer precision than liquid-liquid extraction, which exhibited poor recoveries of less than 86.0%, precisions of less than 5.0% and relative errors of less than 7.0%. The method was used for the determination of meloxicam in healthy human volunteers. |
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Authors:
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Haixia Zhang; Hoo-Kyun Choi |
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Publication Detail:
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Type: Journal Article Date: 2008-08-27 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 392 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-10-21 Completed Date: 2008-12-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 947-53 Citation Subset: IM |
Affiliation:
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College of Pharmacy, Chosun University, 501-759, Gwangju, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acid
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chemistry Acetonitriles / chemistry Adult Anti-Inflammatory Agents, Non-Steroidal / blood* Chromatography, High Pressure Liquid / instrumentation, methods* Detergents / chemistry Drug Stability Humans Piroxicam / analogs & derivatives*, blood Polyethylene Glycols / chemistry Reproducibility of Results Sensitivity and Specificity Solvents / chemistry Thiazines / blood* Thiazoles / blood* |
| Chemical | |
Reg. No./Substance:
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0/Acetonitriles; 0/Anti-Inflammatory Agents, Non-Steroidal; 0/Detergents; 0/Polyethylene Glycols; 0/Solvents; 0/Thiazines; 0/Thiazoles; 36322-90-4/Piroxicam; 59804-37-4/tenoxicam; 64-19-7/Acetic Acid; 71125-38-7/meloxicam; 75-05-8/acetonitrile |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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