Document Detail


Analysis of major caprine milk proteins by reverse-phase high-performance liquid chromatography and electrospray ionization-mass spectrometry.
MedLine Citation:
PMID:  10659957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Major proteins from caprine milk were separated by preparative gel permeation and cation-exchange fast protein liquid chromatography and were characterized by flow injection analysis by electrospray ionization mass spectrometry. In addition, proteins from whole skim milk and whole casein were analyzed by coupling reverse-phase HPLC and electrospray ionization mass spectrometry by two different chromatographic methods. These methods successfully resolved the major caprine milk proteins and main casein variants. The experimental molecular masses of major milk proteins and variants were: 19,302 for kappa-CN 2P; 25,599 for alphas2-CN A-11P; 25,514 for alphas2-CN B-10P; 23,370 for alphas1-CN A-8P; 23,345 for alphas1-CN B-8P; 23,264 for alphas1-CN E-8P; 18,817 for alphas1-CN F-3P; 23,835 for beta-CN 6P; 18,181 for beta-LG; 14,180 for alpha-LA and 66,318 for serum albumin.
Authors:
A J Trujillo; I Casals; B Guamis
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of dairy science     Volume:  83     ISSN:  0022-0302     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-02-17     Completed Date:  2000-02-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  11-9     Citation Subset:  IM    
Affiliation:
Technologia del Aliments, Centre de Referència en Tecnologia dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Spain. Toni.Trujillo@uab.es
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MeSH Terms
Descriptor/Qualifier:
Animals
Caseins / analysis
Chromatography, High Pressure Liquid*
Female
Goats*
Lactalbumin / analysis
Lactoglobulins / analysis
Mass Spectrometry*
Milk Proteins / analysis*
Molecular Weight
Serum Albumin / analysis
Chemical
Reg. No./Substance:
0/Caseins; 0/Lactoglobulins; 0/Milk Proteins; 0/Serum Albumin; 9013-90-5/Lactalbumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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