Document Detail


Analysis of macrophage migration inhibitory factor (MIF) in patients with idiopathic pulmonary fibrosis.
MedLine Citation:
PMID:  19464392     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
By proteomic approach we previously characterised bronchoalveolar lavage (BAL) protein profiles of patients with idiopathic pulmonary fibrosis (IPF), sarcoidosis and systemic sclerosis. Among differently expressed proteins we identified macrophage migration inhibitory factor (MIF), a multi-function pleiotropic cytokine. This study was performed to validate our findings by a further proteomic approach and ELISA in a larger population of patients and controls. MIF expression in lung tissue was also evaluated by immunohistochemistry. MIF was identified in all 2-DE gels of IPF patients and it was significantly increased compared to controls (p<0.05). This result was confirmed by ELISA: MIF concentrations were significantly higher in IPF patients than controls (p<0.001) and were directly correlated with neutrophil percentages (p=0.0095). Immunohistochemical analysis revealed enhanced expression in bronchiolar epithelium, alveolar epithelium, and fibroblastic foci. In conclusion, MIF is a pleiotropic cytokine that could be involved in the pathogenesis of IPF, being particularly abundant in BAL of these patients and mainly expressed in the areas of active fibrosis.
Authors:
E Bargagli; C Olivieri; N Nikiforakis; M Cintorino; B Magi; M G Perari; C Vagaggini; D Spina; A Prasse; P Rottoli
Publication Detail:
Type:  Journal Article     Date:  2009-05-21
Journal Detail:
Title:  Respiratory physiology & neurobiology     Volume:  167     ISSN:  1878-1519     ISO Abbreviation:  Respir Physiol Neurobiol     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-22     Completed Date:  2009-09-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101140022     Medline TA:  Respir Physiol Neurobiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  261-7     Citation Subset:  IM    
Affiliation:
Department of Clinical Medicine and Immunology, Respiratory Diseases Section, Siena University, Viale Bracci 1, Siena, Italy. bargagli2@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Blood Gas Analysis
Bronchoalveolar Lavage Fluid / cytology
Electrophoresis, Gel, Two-Dimensional
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunohistochemistry
Macrophage Migration-Inhibitory Factors / blood*
Male
Middle Aged
Pulmonary Fibrosis / blood*
Respiratory Function Tests
Smoking / adverse effects
Chemical
Reg. No./Substance:
0/Macrophage Migration-Inhibitory Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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