Document Detail


Analysis of lysozyme in cheese by immunocapture mass spectrometry.
MedLine Citation:
PMID:  19709935     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The enzyme lysozyme is used as a preservative to prevent late blowing of ripened cheese, caused by Clostridium tyrobutyricum. Since the enzyme is extracted from hen egg white, lysozyme has to be declared on food product labels as a potential allergen. Here, a method is reported that combines immunocapture purification and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis for the detection of lysozyme in cheese samples. Cheese extracts were treated with magnetic particles coated with a monoclonal antibody directed against lysozyme. After immunocapture purification, lysozyme was detected by MALDI-TOF-MS. The limit of detection of the assay was about 5mg/kg lysozyme in cheese. The method reliably distinguished between cheese samples which had been produced with and without lysozyme. Thus, the novel assay allows the reliable, sensitive, and specific detection of lysozyme in a food matrix. The assay could be easily adapted to other target peptides and proteins in complex food matrices and, therefore, has a broad application potential, e.g. for the analysis of allergens.
Authors:
Nadine Schneider; Cord-Michael Becker; Monika Pischetsrieder
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-07
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  878     ISSN:  1873-376X     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-02-04     Completed Date:  2010-04-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  201-6     Citation Subset:  IM    
Copyright Information:
2009 Elsevier B.V. All rights reserved.
Affiliation:
Department of Chemistry and Pharmacy, Emil Fischer Center, University of Erlangen-Nuremberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / chemistry,  immunology,  metabolism
Cheese / analysis*
Immunosorbent Techniques*
Magnetics
Microspheres
Muramidase / analysis*,  immunology,  metabolism
Peptide Mapping
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; EC 3.2.1.17/Muramidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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