Document Detail

Analysis of lysophosphatidic acid (LPA) receptor and LPA-induced endometrial prostaglandin-endoperoxide synthase 2 expression in the porcine uterus.
MedLine Citation:
PMID:  18703629     Owner:  NLM     Status:  MEDLINE    
Lysophosphatidic acid (LPA), a simple phospholipid-derived mediator with diverse biological actions, acts through the specific G protein-coupled receptors endothelial differentiation gene (EDG) 2, EDG4, EDG7, and GPR23. Recent studies indicate a critical role for LPA receptor signaling in embryo implantation. To understand how LPA acts in the uterus during pregnancy in pigs, we evaluated: 1) spatial and temporal expression of LPA receptors in the uterine endometrium during the estrous cycle and pregnancy and in early-stage concepti, 2) LPA levels in uterine luminal fluids from d 12 of the estrous cycle and pregnancy, 3) effects of steroid hormones on EDG7 mRNA levels, and 4) effects of LPA on prostaglandin-endoperoxide synthase 2 (PTGS2) mRNA levels in the uterine endometrium using explant cultures. Of the four receptors, EDG7 was dominant, and its expression was regulated by pregnancy stage and status. EDG7 expression was highest on d 12 pregnancy, and localized to the luminal and glandular epithelium, and EDG7 mRNA levels were elevated by estrogen in the endometrium. EDG7 expression was also detected in concepti of d 12 and 15. LPA with various fatty acyl groups was present in the uterine lumen on d 12 of both the estrous cycle and pregnancy. LPA increased PTGS2 mRNA abundance in the uterine endometrium. These results indicate that LPA produced in the uterine endometrium may play a critical role in uterine endometrial function and conceptus development through EDG7-mediated PTGS2 expression during implantation and establishment of pregnancy in pigs.
Heewon Seo; Mingoo Kim; Yohan Choi; Chang-Kyu Lee; Hakhyun Ka
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Publication Detail:
Type:  Journal Article     Date:  2008-08-14
Journal Detail:
Title:  Endocrinology     Volume:  149     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-21     Completed Date:  2009-03-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6166-75     Citation Subset:  AIM; IM    
Department of Biological Resources and Technology, Yonsei University, Wonju 220-710, Republic of Korea.
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MeSH Terms
Blotting, Northern
Cyclooxygenase 2 / genetics*
Endometrium / drug effects*,  metabolism
Estrous Cycle / physiology
Gene Expression / drug effects
In Situ Hybridization
Lysophospholipids / metabolism,  pharmacology*
RNA, Messenger / genetics,  metabolism
Random Allocation
Receptors, Estrogen / genetics
Receptors, Lysophosphatidic Acid / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Uterus / drug effects*,  metabolism
Reg. No./Substance:
0/Lysophospholipids; 0/RNA, Messenger; 0/Receptors, Estrogen; 0/Receptors, Lysophosphatidic Acid; 22002-87-5/lysophosphatidic acid; EC 2

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