| Analysis of lysophosphatidic acid (LPA) receptor and LPA-induced endometrial prostaglandin-endoperoxide synthase 2 expression in the porcine uterus. | |
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MedLine Citation:
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PMID: 18703629 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lysophosphatidic acid (LPA), a simple phospholipid-derived mediator with diverse biological actions, acts through the specific G protein-coupled receptors endothelial differentiation gene (EDG) 2, EDG4, EDG7, and GPR23. Recent studies indicate a critical role for LPA receptor signaling in embryo implantation. To understand how LPA acts in the uterus during pregnancy in pigs, we evaluated: 1) spatial and temporal expression of LPA receptors in the uterine endometrium during the estrous cycle and pregnancy and in early-stage concepti, 2) LPA levels in uterine luminal fluids from d 12 of the estrous cycle and pregnancy, 3) effects of steroid hormones on EDG7 mRNA levels, and 4) effects of LPA on prostaglandin-endoperoxide synthase 2 (PTGS2) mRNA levels in the uterine endometrium using explant cultures. Of the four receptors, EDG7 was dominant, and its expression was regulated by pregnancy stage and status. EDG7 expression was highest on d 12 pregnancy, and localized to the luminal and glandular epithelium, and EDG7 mRNA levels were elevated by estrogen in the endometrium. EDG7 expression was also detected in concepti of d 12 and 15. LPA with various fatty acyl groups was present in the uterine lumen on d 12 of both the estrous cycle and pregnancy. LPA increased PTGS2 mRNA abundance in the uterine endometrium. These results indicate that LPA produced in the uterine endometrium may play a critical role in uterine endometrial function and conceptus development through EDG7-mediated PTGS2 expression during implantation and establishment of pregnancy in pigs. |
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Authors:
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Heewon Seo; Mingoo Kim; Yohan Choi; Chang-Kyu Lee; Hakhyun Ka |
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Publication Detail:
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Type: Journal Article Date: 2008-08-14 |
Journal Detail:
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Title: Endocrinology Volume: 149 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-21 Completed Date: 2009-03-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 6166-75 Citation Subset: AIM; IM |
Affiliation:
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Department of Biological Resources and Technology, Yonsei University, Wonju 220-710, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Northern Cyclooxygenase 2 / genetics* Endometrium / drug effects*, metabolism Estrous Cycle / physiology Female Gene Expression / drug effects In Situ Hybridization Lysophospholipids / metabolism, pharmacology* Pregnancy RNA, Messenger / genetics, metabolism Random Allocation Receptors, Estrogen / genetics Receptors, Lysophosphatidic Acid / genetics* Reverse Transcriptase Polymerase Chain Reaction Swine Uterus / drug effects*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Lysophospholipids; 0/RNA, Messenger; 0/Receptors, Estrogen; 0/Receptors, Lysophosphatidic Acid; 22002-87-5/lysophosphatidic acid; EC 1.14.99.1/Cyclooxygenase 2 |
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