| Analysis of losartan and carvedilol in urine and plasma samples using a dispersive liquid-liquid microextraction isocratic HPLC-UV method. | |
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MedLine Citation:
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PMID: 23216121 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background: A simple, precise and sensitive HPLC method has been developed for simultaneous determination of carvedilol and losartan in human plasma and urine samples. The analytes were extracted by a dispersive liquid-liquid microextraction method. A mobile phase of 15 mM sodium dihydrogen phosphate buffer (pH 4.0)/acetonitrile/2-propanol (70/27.5/2.5, v/v/v) was used to separate the drugs using a Waters(®) ODS column (250 × 4.6 mm) and detected by a UV detector at 222 nm. Results: The developed method is selective for studied drugs possessing a linearity range of 0.1-1.0 and 0.05-0.75 µg/ml, respectively, for losartan and carvedilol with precision <15%. The accuracy is better than 15% and the mean recovery of carvedilol and losartan was 98.9 and 100.2% for plasma and 100.7 and 100.5% for urine samples, respectively. Conclusion: The developed method is applicable for therapeutic drug monitoring and PK analyses. |
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Authors:
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Somaieh Soltani; Amir M Ramezani; Naser Soltani; Abolghasem Jouyban |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bioanalysis Volume: 4 ISSN: 1757-6199 ISO Abbreviation: Bioanalysis Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101512484 Medline TA: Bioanalysis Country: England |
Other Details:
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Languages: eng Pagination: 2805-21 Citation Subset: IM |
Affiliation:
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Cardiovascular Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran. |
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Descriptor/Qualifier:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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