Document Detail


Analysis of losartan and carvedilol in urine and plasma samples using a dispersive liquid-liquid microextraction isocratic HPLC-UV method.
MedLine Citation:
PMID:  23216121     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: A simple, precise and sensitive HPLC method has been developed for simultaneous determination of carvedilol and losartan in human plasma and urine samples. The analytes were extracted by a dispersive liquid-liquid microextraction method. A mobile phase of 15 mM sodium dihydrogen phosphate buffer (pH 4.0)/acetonitrile/2-propanol (70/27.5/2.5, v/v/v) was used to separate the drugs using a Waters® ODS column (250 × 4.6 mm) and detected by a UV detector at 222 nm.
RESULTS: The developed method is selective for studied drugs possessing a linearity range of 0.1-1.0 and 0.05-0.75 µg/ml, respectively, for losartan and carvedilol with precision <15%. The accuracy is better than 15% and the mean recovery of carvedilol and losartan was 98.9 and 100.2% for plasma and 100.7 and 100.5% for urine samples, respectively.
CONCLUSION: The developed method is applicable for therapeutic drug monitoring and PK analyses.
Authors:
Somaieh Soltani; Amir M Ramezani; Naser Soltani; Abolghasem Jouyban
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioanalysis     Volume:  4     ISSN:  1757-6199     ISO Abbreviation:  Bioanalysis     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  2013-05-09     Revised Date:  2013-05-28    
Medline Journal Info:
Nlm Unique ID:  101512484     Medline TA:  Bioanalysis     Country:  England    
Other Details:
Languages:  eng     Pagination:  2805-21     Citation Subset:  IM    
Affiliation:
Cardiovascular Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
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MeSH Terms
Descriptor/Qualifier:
Antihypertensive Agents / analysis*,  isolation & purification,  pharmacokinetics
Carbazoles / analysis*,  blood,  urine
Chromatography, High Pressure Liquid*
Half-Life
Humans
Hydrogen-Ion Concentration
Liquid Phase Microextraction
Losartan / analysis*,  blood,  urine
Propanolamines / analysis*,  blood,  urine
Salts / chemistry
Solvents / chemistry
Spectrophotometry, Ultraviolet*
Urinalysis / instrumentation,  methods*
Chemical
Reg. No./Substance:
0/Antihypertensive Agents; 0/Carbazoles; 0/Propanolamines; 0/Salts; 0/Solvents; 0K47UL67F2/carvedilol; 114798-26-4/Losartan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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