Document Detail


Analysis of lipids by gas-liquid chromatography and complementary methods in four strains of Aedes aegypti mosquitoes.
MedLine Citation:
PMID:  4017543     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fatty acid composition of total lipids, neutral lipids and phospholipids of strains of Aedes aegypti were determined. The fatty acid composition of the strains differed quantitatively with regard to the relative percentage of commonly occurring fatty acids. Gas-liquid chromatography of fatty acid methyl esters showed 18:1 (oleic or elaidic) to be the predominant fatty acid. The fatty acid was identified as oleic by argentation thin-layer chromatography. A modified colorimetric method was used to determine tissue-free fatty acids. The lipids were predominantly triacylglycerol with lesser amounts of free fatty acids and decreasing amount of sterol ester, sterol, monoacylglycerol, diacylglycerol and hydrocarbons. The data show considerable lipid differences between the Caribbean strains (Les Cayes, Haiti, and San Juan, Puerto Rico) and the Jakarta (Indonesia) strain. The Shimba Hills (Kenya) strain was more similar to Jakarta than to the Caribbean strains. The results obtained with the different strains are discussed in relation to the established oral susceptibility to Dengue 1 and Dengue 2, yellow fever, and genetic analysis by isoenzyme studies.
Authors:
S Miller; R J Novak
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Comparative biochemistry and physiology. B, Comparative biochemistry     Volume:  81     ISSN:  0305-0491     ISO Abbreviation:  Comp. Biochem. Physiol., B     Publication Date:  1985  
Date Detail:
Created Date:  1985-09-04     Completed Date:  1985-09-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984730R     Medline TA:  Comp Biochem Physiol B     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  235-40     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aedes / analysis*
Animals
Chromatography, Gas / methods
Chromatography, Thin Layer
Fatty Acids / analysis
Fatty Acids, Nonesterified / analysis
Glycerides / analysis
Lipids / analysis*
Phospholipids / analysis
Species Specificity
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Glycerides; 0/Lipids; 0/Phospholipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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