| Analysis of ligand-dependent recruitment of coactivator peptides to RXRbeta in a time-resolved fluorescence resonance energy transfer assay. | |
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MedLine Citation:
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PMID: 17184907 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Because RXR plays a significant role in nuclear receptor signaling as a common heterodimeric partner for TR, PPAR, RAR, VDR, LXR and others, the ability of RXRbeta ligand binding domain (LBD) to interact with coregulator peptides bearing LXXLL or other interaction motifs was investigated using time-resolved fluorescence resonance energy transfer (TR-FRET). The random phage display peptide D22 and peptides derived from PGC1alpha, SRC1-4, SRC2-3, PRIP/RAP250 and RIP140 yielded the highest TR-FRET signal with RXRbeta LBD in the presence of saturating 9-cis retinoic acid (9-cisRA). Several peptides including D22, PGC1alpha, SRC3-2, PRIP/RAP250 and SRC1-4 also formed a complex with RXRbeta LBD in the presence of all-trans retinoic acid (at-RA) and the fatty acids, phytanic acid (PA) and docosahexaenoic acid (DHA). Determination of the dose dependency (EC50) of these compounds to recruit D22 to RXRbeta LBD indicated that the rank order potency was 9-cisRA>PA>at-RA>DHA. The ligands 9-cisRA and at-RA yielded an overall higher fold-change in D22 recruitment to RXRbeta LBD suggesting that more RXRbeta LBD-D22 complex was formed in the presence of these ligands under the assay conditions tested. The statistical parameter Z' factor for 9-cisRA-induced recruitment of D22 to RXRbeta LBD was 0.6 after 2h incubation, indicating a robust methodology that could be applied to high throughput screening. These results demonstrate that RXRbeta occupied with the fatty acid ligands, DHA and PA, can recruit coactivator peptides in a ligand-dependent manner. |
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Authors:
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Deborah K Stafslien; Kevin L Vedvik; Therese De Rosier; Mary Szatkowski Ozers |
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Publication Detail:
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Type: Journal Article Date: 2006-12-20 |
Journal Detail:
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Title: Molecular and cellular endocrinology Volume: 264 ISSN: 0303-7207 ISO Abbreviation: Mol. Cell. Endocrinol. Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2007-01-22 Completed Date: 2007-03-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: Ireland |
Other Details:
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Languages: eng Pagination: 82-9 Citation Subset: IM |
Affiliation:
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Invitrogen Discovery Sciences, Madison, Wisconsin 53719, USA. Deb.Stafslien@invitrogen.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptor Proteins, Signal Transducing
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chemistry*,
metabolism Amino Acid Motifs Fluorescence Resonance Energy Transfer* Humans Kinetics Ligands Peptides / chemistry* Protein Binding / physiology Protein Structure, Tertiary / physiology Retinoid X Receptor beta / chemistry*, metabolism Signal Transduction / drug effects, physiology Tretinoin / chemistry, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Proteins, Signal Transducing; 0/Ligands; 0/Peptides; 0/Retinoid X Receptor beta; 302-79-4/Tretinoin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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