| Analysis of lentiviral genomic variation by denaturing gradient gel electrophoresis. | |
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MedLine Citation:
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PMID: 7980942 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Retroviruses are known for their genetic variability. In any infection, several genotypes usually exist within the host. We have used denaturing gradient gel electrophoresis to study genetic variation of ovine lentiviruses. Starting with viral DNA from cells infected in vitro, a portion of the envelope gene was amplified by PCR, and the products were analyzed by DGGE. With this technique we have been able to detect sequence variations between and within virus isolates and to show evolution of the predominant viral species upon in vivo passage. |
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Authors:
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T M Woodward; J Carlson; C McClelland; J C DeMartini |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: BioTechniques Volume: 17 ISSN: 0736-6205 ISO Abbreviation: BioTechniques Publication Date: 1994 Aug |
Date Detail:
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Created Date: 1995-01-05 Completed Date: 1995-01-05 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8306785 Medline TA: Biotechniques Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 366-71 Citation Subset: IM |
Affiliation:
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Colorado State University, College of Veterinary Medicine and Biomedical Sciences, Fort Collins. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Electrophoresis Genetic Variation* Genome, Viral* Lentiviruses, Ovine-Caprine / genetics* Molecular Sequence Data Polymerase Chain Reaction |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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