Document Detail


Analysis of latent viral gene expression in natural and experimental latency models of human cytomegalovirus and its correlation with histone modifications at a latent promoter.
MedLine Citation:
PMID:  19906945     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human cytomegalovirus (HCMV) is an opportunistic human pathogen that establishes a lifelong latent infection, which can reactivate periodically. If unchecked by a robust immune response, this reactivation can result in severe disease in immunocompromised patients. Reactivation of latent virus in myeloid progenitor cells is concomitant with cellular differentiation through regulation of the major immediate-early promoter (MIEP) by chromatin remodelling. In this study, we analysed the expression of the latent gene transcript UL81-82as (LUNA). LUNA is expressed in latently infected CD34(+) cells and its expression decreases as CD34(+) cells differentiate to immature dendritic cells. Upon maturation (and HCMV reactivation), a second wave of transcription occurs, consistent with expression during lytic infection. Furthermore, we show that the LUNA promoter is associated with acetylated histones during HCMV latency in experimentally and naturally infected CD34(+) cells, thus suggesting that latent gene promoters are, like the MIEP, regulated by post-translational modifications of their associated histone proteins.
Authors:
Matthew B Reeves; John H Sinclair
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-11
Journal Detail:
Title:  The Journal of general virology     Volume:  91     ISSN:  1465-2099     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-12     Completed Date:  2010-03-02     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  599-604     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Cell Differentiation
Cytomegalovirus / genetics,  physiology*
Gene Expression Profiling
Gene Expression Regulation, Viral*
Histones / metabolism*
Humans
Myeloid Cells / virology
Promoter Regions, Genetic*
Protein Processing, Post-Translational
Stem Cells / virology
Virus Latency*
Grant Support
ID/Acronym/Agency:
G0701279//Medical Research Council; G9202171//Medical Research Council; //Medical Research Council
Chemical
Reg. No./Substance:
0/Histones

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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