Document Detail

Analysis of isoflavonoids from leguminous plant extracts by RPHPLC/DAD and electrospray ionization mass spectrometry.
MedLine Citation:
PMID:  17469767     Owner:  NLM     Status:  MEDLINE    
Traditionally, food is associated with energetic and nutritious characteristics such as sources of carbohydrates, proteins and lipids. Recently, however, foods with bioactive substances such as isoflavones have received great attention. The objective of this study was therefore to evaluate the presence of the isoflavones daidzein, glycitein, genistein and their conjugated forms in grains and leaves of several leguminous plants utilized largely in Brazilian cuisine. Grains used in Peruvian cuisine were also analyzed. After extracting phenolic compounds with methanol (80%), isoflavones as detected by reversed-phase high-performance liquid chromatography/ diode-array detector were only found in chickpeas and soybean. Chickpea extracts showed only the isoflavone genistein at 31 microg/g defatted flour. Detection of these isoflavones was confirmed by electrospray ionization mass and tandem mass spectrometric experiments. For soybean, a distinct distribution of isoflavones was found in hypocotyls and cotyledon. The highest concentration of isoflavones found was approximately 5.9 mg/g for hypocotyls, whereas the total concentration of isoflavone was around 0.4 mg/g for the cotyledons (dry matter). These results indicate that isoflavone concentrations vary within the different tissues of the leguminous species tested.
Claudio L Aguiar; Antonio S Baptista; Severino M Alencar; Renato Haddad; Marcos N Eberlin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of food sciences and nutrition     Volume:  58     ISSN:  0963-7486     ISO Abbreviation:  Int J Food Sci Nutr     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-05-01     Completed Date:  2007-09-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9432922     Medline TA:  Int J Food Sci Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  116-24     Citation Subset:  IM    
Fazenda Experimental UNOPAR, Universidade Norte do Paraná (UNOPAR), Tamarana/PR, Brazil.
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MeSH Terms
Chromatography, High Pressure Liquid / methods
Fabaceae / chemistry*
Food Analysis / methods
Genistein / analysis
Isoflavones / analysis*
Spectrometry, Mass, Electrospray Ionization / methods
Reg. No./Substance:
0/Isoflavones; 40957-83-3/glycitein; 446-72-0/Genistein; 486-66-8/daidzein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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