| Analysis of interleukin-1alpha (IL-1alpha) and interleukin-8 (IL-8) expression and release in in vitro reconstructed human epidermis for the prediction of in vivo skin irritation and/or sensitization. | |
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MedLine Citation:
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PMID: 12781210 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present study, reconstructed human epidermis (RHE) was used as an in vitro model to discriminate 1-chloro-2,4-dinitrobenzene (DNCB), nickel sulfate (NiSO(4)), oxazolone (OXA), 2,4-dinitrofluorobenzene (DNFB) and 2,4,6-trinitrobenzenesulfonic acid (TNBS) as skin sensitizers from benzalkonium chloride (BC), benzoic acid (BA) and sodium lauryl sulfate (SLS) as skin irritants. Our criteria were (a) the differential IL-1alpha and IL-8 synthesis and release (b) cytotoxicity assessment by MTT assay. When the RHE are topically treated with the sensitizers, very low levels of extra- and intracellular IL-1alpha are observed although they induce significant cytotoxicity. In contrast, they exhibit a sharp maximum of IL-8 release. In the presence of the tested irritants, we observe the inverse cytokine release profile, although they induce dose-dependent cytotoxicity profiles similar to those observed with the sensitizers. Finally, IL-1alpha mRNA upregulation is observed after topical application of both sensitizers and irritants, but only the latter significantly increase extracellular IL-1alpha. In conclusion, our results suggest that the associated determination of IL-8, with IL-1alpha, and MTT conversion are at least necessary to discriminate and classify, in a single assay, irritant and sensitizing agents and represent a potential in vitro alternative to two classical in vivo assays. |
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Authors:
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A Coquette; N Berna; A Vandenbosch; M Rosdy; B De Wever; Y Poumay |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 17 ISSN: 0887-2333 ISO Abbreviation: Toxicol In Vitro Publication Date: 2003 Jun |
Date Detail:
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Created Date: 2003-06-03 Completed Date: 2004-01-28 Revised Date: 2009-04-10 |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 311-21 Citation Subset: IM |
Affiliation:
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Department of Biology, SGS Biopharma S.A., Vieux Chemin du Poète 10, B-1301, Wavre, Belgium. alain_coquette@sgs.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Topical Animal Testing Alternatives Biological Assay / methods Biological Markers / analysis* Coloring Agents / administration & dosage Culture Techniques Epidermis / cytology, drug effects* Humans Interleukin-1 / biosynthesis*, secretion Interleukin-8 / biosynthesis*, secretion Irritants / toxicity* Predictive Value of Tests Skin Irritancy Tests / methods Tetrazolium Salts / administration & dosage Thiazoles / administration & dosage |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Coloring Agents; 0/Interleukin-1; 0/Interleukin-8; 0/Irritants; 0/Tetrazolium Salts; 0/Thiazoles; 298-93-1/thiazolyl blue |
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