Document Detail

Analysis of the interaction between an alpha (1----6)dextran-specific mouse hybridoma antibody and dextran B512 by affinity electrophoresis.
MedLine Citation:
PMID:  1381368     Owner:  NLM     Status:  MEDLINE    
Carbohydrates are common environmental antigens. As dextran B512 is composed of a repeating structure of simple antigenic determinants, it is widely used to study the immunochemical properties of immunoglobulins. Two-dimensional affinity electrophoresis patterns of a mouse monoclonal antidextran antibody (35.8.2H; IgG1, BALB/c) were produced to obtain insights into the microheterogeneity of the monoclonal antibody. The monoclonal antibody was separated into about six spots which had an identical affinity to dextran B512, but differed in their isoelectric points (pI). In addition, the pH dependence of the binding affinity of this antidextran to dextran B512 was examined. By comparing affinities obtained by affinity electrophoresis between weakly basic (pH 9.5) and weakly acidic (pH 3.8) discontinuous buffer systems, the latter showed an affinity about 500 times lower than the former. The change in the affinity was investigated with a continuous pH gradient by an affinity titration curve and was seen to change markedly at about pH 6. This suggests that the histidine at residue 34 in the light-chain CDR1 is largely responsible for the dextran binding.
Y Mimura; K Nakamura; K Takeo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  597     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-10-06     Completed Date:  1992-10-06     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  345-50     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Antibodies / analysis*
Antibodies, Monoclonal / analysis,  immunology
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Hybridomas / immunology*
Hydrogen-Ion Concentration
Isoelectric Focusing
Mice, Inbred BALB C
Mice, Inbred Strains
Reg. No./Substance:
0/Antibodies; 0/Antibodies, Monoclonal; K3R6ZDH4DU/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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