Document Detail


Analysis of the interaction between an alpha (1----6)dextran-specific mouse hybridoma antibody and dextran B512 by affinity electrophoresis.
MedLine Citation:
PMID:  1381368     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Carbohydrates are common environmental antigens. As dextran B512 is composed of a repeating structure of simple antigenic determinants, it is widely used to study the immunochemical properties of immunoglobulins. Two-dimensional affinity electrophoresis patterns of a mouse monoclonal antidextran antibody (35.8.2H; IgG1, BALB/c) were produced to obtain insights into the microheterogeneity of the monoclonal antibody. The monoclonal antibody was separated into about six spots which had an identical affinity to dextran B512, but differed in their isoelectric points (pI). In addition, the pH dependence of the binding affinity of this antidextran to dextran B512 was examined. By comparing affinities obtained by affinity electrophoresis between weakly basic (pH 9.5) and weakly acidic (pH 3.8) discontinuous buffer systems, the latter showed an affinity about 500 times lower than the former. The change in the affinity was investigated with a continuous pH gradient by an affinity titration curve and was seen to change markedly at about pH 6. This suggests that the histidine at residue 34 in the light-chain CDR1 is largely responsible for the dextran binding.
Authors:
Y Mimura; K Nakamura; K Takeo
Related Documents :
20954248 - Calculating pka values in the camp-dependent protein kinase: the effect of conformation...
16141328 - Targeting of the fyve domain to endosomal membranes is regulated by a histidine switch.
6313088 - Identification of two mitochondrial gdp-binding sites in rat brown adipose tissue.
7843408 - Specificity of the purified inositol (1,3,4,5) tetrakisphosphate-binding protein from p...
23214628 - Influence of decoys on the noise and dynamics of gene expression.
3804998 - Identification of a new protein synthesis initiation factor from wheat germ.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  597     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-10-06     Completed Date:  1992-10-06     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  345-50     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies / analysis*
Antibodies, Monoclonal / analysis,  immunology
Chromatography, Affinity
Dextrans
Electrophoresis, Polyacrylamide Gel
Hybridomas / immunology*
Hydrogen-Ion Concentration
Isoelectric Focusing
Mice
Mice, Inbred BALB C
Mice, Inbred Strains
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antibodies, Monoclonal; K3R6ZDH4DU/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  CD36 peptides enhance or inhibit CD36-thrombospondin binding. A two-step process of ligand-receptor ...
Next Document:  Cellulose bearing covalently linked copper phthalocyanine trisulphonate as an adsorbent selective fo...